Spatial Distribution Patterns of Root-Associated Bacterial Communities Mediated by Root Exudates in Different Aged Ratooning Tea Monoculture Systems
Abstract
:1. Introduction
2. Results
2.1. The Problems of Camellia sinensis (L.) Plantation under Continuous Monoculture
2.2. Soil Physio-Chemical Properties and Root Exudates
2.3. 16S rDNA-Based Meta-Genomic Analysis of Tea Root-Associated Bacteria
2.4. Distinct and Overlapping Bacterial Communities of Root-Associated Bacterial Communities across Different Tea Plantations of Different Ages
2.5. Interactions of Bacterial Abundance with Soil Physio-Chemical Properties and Root Exudates
3. Discussion
4. Materials and Methods
4.1. The Collection of Plant and Rhizosphere Soil Samples
4.2. Quality Parameters and Soil Properties Determination
4.3. Identification and Quantification of Allelochemicals from Tea Root Exudates
4.4. DNA Extraction and Purification
4.5. The Metagenomic Analysis of the Root-Associated Rhizo-Compartment Bacteria
4.6. Statistical and Bioinformatics Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Treatments | TNN (g/kg) | TPY (mg/kg) | TPP (g/kg) | TAA (g/kg) | WC (%) |
---|---|---|---|---|---|
2YTL | 1.70 a | 0.12 a | 106.40 a | 12.44 a | 0.76 a |
30YTL | 0.47 b | 0.02 b | 30.66 b | 8.87 b | 0.56 a |
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Arafat, Y.; Wei, X.; Jiang, Y.; Chen, T.; Saqib, H.S.A.; Lin, S.; Lin, W. Spatial Distribution Patterns of Root-Associated Bacterial Communities Mediated by Root Exudates in Different Aged Ratooning Tea Monoculture Systems. Int. J. Mol. Sci. 2017, 18, 1727. https://doi.org/10.3390/ijms18081727
Arafat Y, Wei X, Jiang Y, Chen T, Saqib HSA, Lin S, Lin W. Spatial Distribution Patterns of Root-Associated Bacterial Communities Mediated by Root Exudates in Different Aged Ratooning Tea Monoculture Systems. International Journal of Molecular Sciences. 2017; 18(8):1727. https://doi.org/10.3390/ijms18081727
Chicago/Turabian StyleArafat, Yasir, Xiaoya Wei, Yuhang Jiang, Ting Chen, Hafiz Sohaib Ahmed Saqib, Sheng Lin, and Wenxiong Lin. 2017. "Spatial Distribution Patterns of Root-Associated Bacterial Communities Mediated by Root Exudates in Different Aged Ratooning Tea Monoculture Systems" International Journal of Molecular Sciences 18, no. 8: 1727. https://doi.org/10.3390/ijms18081727