Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets
Abstract
:1. Introduction
2. Results
2.1. Transforming Growth Factor Beta 1 (TGF-β1) Induces Epithelial-to-Mesenchymal Transition (EMT) in C4-I and HTB-35 Cells
2.2. CA Attenuates the Migratory Capacity of C4-I and Met Inhibits Motility of HTB-35 Cells
2.3. CA and Met Treatment of C4-I Cells Increases Epithelial Adhesive Markers and Decreases Mesenchymal Transcription Factors Regulating EMT
2.4. CA Downregulate the Expression of MMP-9 and Specific Tissue Inhibitor of Matrix Metalloproteinases TIMP-1 in C4-I Cells
2.5. Met Attenuates Mesenchymal Marker of Malignant HTB-35 Cells
2.6. Met Inhibits the Expression of CAIX in HTB-35 Cells under Hypoxic Conditions
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. Immunoblotting
4.3. Quantitative Polymerase Chain Reaction (qPCR)
4.4. Wound Healing Migration
4.5. Hypoxia Conditions
4.6. Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tyszka-Czochara, M.; Lasota, M.; Majka, M. Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets. Int. J. Mol. Sci. 2018, 19, 266. https://doi.org/10.3390/ijms19010266
Tyszka-Czochara M, Lasota M, Majka M. Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets. International Journal of Molecular Sciences. 2018; 19(1):266. https://doi.org/10.3390/ijms19010266
Chicago/Turabian StyleTyszka-Czochara, Malgorzata, Malgorzata Lasota, and Marcin Majka. 2018. "Caffeic Acid and Metformin Inhibit Invasive Phenotype Induced by TGF-β1 in C-4I and HTB-35/SiHa Human Cervical Squamous Carcinoma Cells by Acting on Different Molecular Targets" International Journal of Molecular Sciences 19, no. 1: 266. https://doi.org/10.3390/ijms19010266