The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS
Abstract
:1. Introduction
2. Results
2.1. Salt Induces the Expression of OsVTC1-3 in Rice Root
2.2. OsVTC1-3 Plays a Key Role in the Salt Stress Response of Rice
2.3. Inhibiting the Expression of OsVTC1-3 Decreases the Ability of Rice Roots to Scavenge ROS under Salt Stress
2.4. AsA Plays an Important Role in OsVTC1-3 Regulating Salt Response in Rice Root
2.5. OsVTC1-3 Regulates AsA Synthesis in Rice Root under Salt Stress
3. Discussion
4. Materials and Methods
4.1. The Cultivation of Plant Materials
4.2. The Generation of Transgenic Rice
4.3. The Analysis of OsVTC1-3 Expression by qPCR
4.4. The Determination of the Content of AsA
4.5. The Identification of the Salt Tolerance of Rice Materials
4.6. The Determination of O2− Content in Rice by NBT Staining
4.7. The Determination of H2O2 Content
4.8. GUS Staining of Rice Tissue
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Wang, Y.; Zhao, H.; Qin, H.; Li, Z.; Liu, H.; Wang, J.; Zhang, H.; Quan, R.; Huang, R.; Zhang, Z. The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS. Int. J. Mol. Sci. 2018, 19, 3347. https://doi.org/10.3390/ijms19113347
Wang Y, Zhao H, Qin H, Li Z, Liu H, Wang J, Zhang H, Quan R, Huang R, Zhang Z. The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS. International Journal of Molecular Sciences. 2018; 19(11):3347. https://doi.org/10.3390/ijms19113347
Chicago/Turabian StyleWang, Yayun, Hui Zhao, Hua Qin, Zixuan Li, Hai Liu, Juan Wang, Haiwen Zhang, Ruidang Quan, Rongfeng Huang, and Zhijin Zhang. 2018. "The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS" International Journal of Molecular Sciences 19, no. 11: 3347. https://doi.org/10.3390/ijms19113347