The Mitochondrial Genes BAK1, FIS1 and SFN are Linked with Alterations in Mitochondrial Membrane Potential in Barrett’s Esophagus
Abstract
:1. Introduction
2. Results
2.1. In Vitro Screening Using Human PCR Mitochondrial Gene Microarrays
2.2. In Vivo Validation of Gene Targets
2.3. Functional Effect of BAK1, FIS1 and SFN siRNA Knockdown on Reactive Oxygen Species (ROS) Production, Mitochondrial Mass and Mitochondrial Membrane Potential (MMP) In Vitro
2.4. Assessing the Effect of BAK1, FIS1 and SFN siRNA Knockdown on Cellular Metabolism In Vitro
3. Discussion
4. Materials and Methods
4.1. Ethics
4.2. Metaplastic, Dysplastic and Adenocarcinogenic Cell Lines
4.3. Screening via qRT-PCR Microarray Analysis
4.4. In Vitro qRT-PCR Validation of Gene Targets
4.5. In Vivo qRT-PCR Validation of Gene Targets
4.6. In Vitro siRNA Knockdown of Gene Targets
4.7. Exploring the Functional Effect of siRNA Knockdown on Reactive Oxygen Species Production, Mitochondrial Mass and Mitochondrial Membrane Potential In Vitro
4.8. Characterizing the Metabolic Effect of siRNA Knockdown of BAK1, FIS1 and SFN Utilizing the Seahorse XFE24 Analyzer In Vitro
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Phelan, J.J.; MacCarthy, F.; O’Toole, D.; Ravi, N.; Reynolds, J.V.; O’Sullivan, J. The Mitochondrial Genes BAK1, FIS1 and SFN are Linked with Alterations in Mitochondrial Membrane Potential in Barrett’s Esophagus. Int. J. Mol. Sci. 2018, 19, 3483. https://doi.org/10.3390/ijms19113483
Phelan JJ, MacCarthy F, O’Toole D, Ravi N, Reynolds JV, O’Sullivan J. The Mitochondrial Genes BAK1, FIS1 and SFN are Linked with Alterations in Mitochondrial Membrane Potential in Barrett’s Esophagus. International Journal of Molecular Sciences. 2018; 19(11):3483. https://doi.org/10.3390/ijms19113483
Chicago/Turabian StylePhelan, James J., Finbar MacCarthy, Dermot O’Toole, Narayanasamy Ravi, John V. Reynolds, and Jacintha O’Sullivan. 2018. "The Mitochondrial Genes BAK1, FIS1 and SFN are Linked with Alterations in Mitochondrial Membrane Potential in Barrett’s Esophagus" International Journal of Molecular Sciences 19, no. 11: 3483. https://doi.org/10.3390/ijms19113483