Visfatin Promotes Wound Healing through the Activation of ERK1/2 and JNK1/2 Pathway
Abstract
:1. Introduction
2. Results
2.1. Visfatin Enhances the Proliferation and Migration of HDFs
2.2. Involvement of VEGF in Visfatin-Mediated Wound Healing
2.3. Wound Healing Is Induced by Visfatin via ERK1/2 and JNK1/2 Activation in HDFs
2.4. Wound Healing Activity Induced by Visfatin in Keratinocytes
2.5. Induction of Wound Healing by Visfatin In Vivo
3. Discussion
4. Materials and Methods
4.1. Mice and Cell Culture
4.2. Reagents and Antibody
4.3. cDNA Microarray
4.4. MTT and BrdU Proliferation Assays In Vitro
4.5. Migration Assay In Vitro
4.6. Wound Healing Assay In Vivo
4.7. Histologic Analysis of Wounds and Immunofluorescence Microscopy
4.8. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Real-Time Quantitative PCR (Q-PCR) Analysis
4.9. Western Blot Analysis
4.10. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
hCOL1A1 | CATGACCGAGACGTGTGGAA | GGCAGTTCTTGGTCTCGTCA |
hCOL3A1 | TGTTCCAGGAGCTAAAGGCG | CTCCTGGGATGCCATTTGGT |
hCOL4A1 | TTTTGTGATGCACACCAGCG | AGTAATTGCAGGTCCCACGG |
hCOL5A1 | AGACATGGGCATCAAGGGTG | CCGAGTTTTCCCTTCTCCCC |
hMMP2 | CCCTGTGTCTTCCCCTTCAC | GTAGTTGGCTGTGGTCG |
hMMP9 | GGACAAGCTCTTCGGCTTCT | TCGCTGGTACAGGTCGAGTA |
hFGF2 | AAAAACGGGGGCTTCTTCCT | AGCCAGGTAACGGTTAGCAC |
hFGF7 | TGGATCCTGCCAACTTTGCT | TTCTTGTGTGTCGCTCAGGG |
hFGF10 | GGAGCTACAATCACCT | ACGGGCAGTTCTCCTTCTTG |
hEGF | AGTCCGTGACTTGCAAGAGG | CCTCTTCTTCCCTAGCCCCT |
hTGFβ | TGGTGGAAACCCACAACGAA | GAGCAACACGGGTTCAGGTA |
hCTGF | GTTTGGCCCAGACCCAACTA | GGCTCTGCTTCTCTAGCCTG |
hVEGF | CTTGCCTTGCTGCTCTACCT | GCAGTAGCTGCGCTGATAGA |
hVisfatin | TCGGTTCTGGTGGAGGTTTG | TTGGGATCAGCAACTGGGTC |
hVEGFR1 | GGGGGAAGCAGCCCATAAAT | GCCAGTGTGGTTTGCTTGAG |
hVEGFR2 | CGGTCAACAAAGTCGGGAGA | CAGTGCACCACAAAGACACG |
hGAPDH | TCCTGCACCACCAACTGTT | GTCCACTGTCTTCTGGGTGG |
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Lee, B.-C.; Song, J.; Lee, A.; Cho, D.; Kim, T.S. Visfatin Promotes Wound Healing through the Activation of ERK1/2 and JNK1/2 Pathway. Int. J. Mol. Sci. 2018, 19, 3642. https://doi.org/10.3390/ijms19113642
Lee B-C, Song J, Lee A, Cho D, Kim TS. Visfatin Promotes Wound Healing through the Activation of ERK1/2 and JNK1/2 Pathway. International Journal of Molecular Sciences. 2018; 19(11):3642. https://doi.org/10.3390/ijms19113642
Chicago/Turabian StyleLee, Byung-Cheol, Jisun Song, Arim Lee, Daeho Cho, and Tae Sung Kim. 2018. "Visfatin Promotes Wound Healing through the Activation of ERK1/2 and JNK1/2 Pathway" International Journal of Molecular Sciences 19, no. 11: 3642. https://doi.org/10.3390/ijms19113642