Sub-Toxic Human Amylin Fragment Concentrations Promote the Survival and Proliferation of SH-SY5Y Cells via the Release of VEGF and HspB5 from Endothelial RBE4 Cells
Abstract
:1. Introduction
2. Results
2.1. Time Course of In Vitro hA17–29 Aggregation
2.2. Effect of hA17–29 Fragment on RBE4 Cell Viability and Release in the Medium of Potentially Protective Proteins
2.3. CM Derived from RBE4 Cells Counteracts SH-SY5Y Amyloid-Induced Toxicity
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Peptide Fragments Monomerization and Aggregation Studies
4.3. Rat Brain Endothelial (RBE4) and Human Neuroblastoma (SH-SY5Y) Cell Cultures
4.4. RBE4 Cells Stimulation and Quantification of HspB5 and VEGF in Conditioned Medium
4.5. RBE4 Conditioned Media Counteract SH-SY5Y Amyloid-Induced Toxicity: the Role of VEGF
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cell Treatment | Absorbance at 569 nm (%) in Absence of Anti-Flk1 | Absorbance at 569 nm (%) in Presence of Anti-Flk1 | Difference |
---|---|---|---|
CM-RBE4 | 134.27 (5.98) | 116.55 a (5.23) | −17.72 |
CM-RBE4-hA17–29 | 158.88 (8.18) | 129.47 b (6.99) | −29.41 |
hA17–29 + CM-RBE4 | 113.79 (4.75) | 98.90 d (4.18) | −14.88 |
hA17–29 + CM-RBE4-hA17–29 | 126.38 (7.22) | 101.03 c,e (3.75) | −25.35 |
Cell Treatment | VEGF Levels (pg/mL) | % of Increase Compared to CM-RBE4 |
---|---|---|
CM-RBE4 | 301.75 ± 47.72 | // |
CM-RBE4-hA17–29 | 862.25 ± 72.2 a,b | +286 |
CM-RBE4-Aβ25–35 | 709.50 ± 82.20 a | +235 |
Cell Treatment | LDH Release (% of Controls) in Absence of Anti-Flk1 | LDH Release (% of Controls) in Presence of Anti-Flk1 |
---|---|---|
hA17–29 | 128.98 a (3.82) | NA |
CM-RBE4 | 97.90 (2.31) | 102.75 (4.41) |
CM-RBE4-hA17–29 | 99.97 (1.97) | 100.84 (2.73) |
hA17–29 + CM-RBE4 | 111.92 b (2.54) | 116.09 (3.35) |
hA17–29 + CM-RBE4-hA17–29 | 106.52 b (3.84) | 113.84 (5.18) |
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Caruso, G.; Fresta, C.G.; Lazzarino, G.; Distefano, D.A.; Parlascino, P.; Lunte, S.M.; Lazzarino, G.; Caraci, F. Sub-Toxic Human Amylin Fragment Concentrations Promote the Survival and Proliferation of SH-SY5Y Cells via the Release of VEGF and HspB5 from Endothelial RBE4 Cells. Int. J. Mol. Sci. 2018, 19, 3659. https://doi.org/10.3390/ijms19113659
Caruso G, Fresta CG, Lazzarino G, Distefano DA, Parlascino P, Lunte SM, Lazzarino G, Caraci F. Sub-Toxic Human Amylin Fragment Concentrations Promote the Survival and Proliferation of SH-SY5Y Cells via the Release of VEGF and HspB5 from Endothelial RBE4 Cells. International Journal of Molecular Sciences. 2018; 19(11):3659. https://doi.org/10.3390/ijms19113659
Chicago/Turabian StyleCaruso, Giuseppe, Claudia G. Fresta, Giacomo Lazzarino, Donatella A. Distefano, Paolo Parlascino, Susan M. Lunte, Giuseppe Lazzarino, and Filippo Caraci. 2018. "Sub-Toxic Human Amylin Fragment Concentrations Promote the Survival and Proliferation of SH-SY5Y Cells via the Release of VEGF and HspB5 from Endothelial RBE4 Cells" International Journal of Molecular Sciences 19, no. 11: 3659. https://doi.org/10.3390/ijms19113659