Expression, Localization, and Activity of the Aryl Hydrocarbon Receptor in the Human Placenta
Abstract
:1. Introduction
2. Results
2.1. AhR Expression in Human Placenta During Ontogeny
2.2. AhR Localization in Human Placental Chorionic Villi During Ontogeny
2.3. AhR Expression, Localization, and Activity in Human Purified Trophoblasts
2.4. AhR Expression, Localization, and Activity in the BeWo Trophoblast Model
2.5. Effect of Benzo-(a)-Pyrene on AhR Activity in the BeWo Model
3. Discussion
4. Materials & Methods
4.1. Placentae Collection and Chorionic Villi Isolation
4.2. Explants
4.3. Cell Culture
4.4. Other Cell Types
4.5. Cell and Explant Incubation
4.6. RNA Extraction, Reverse Transcription, and Quantitative Real-Time PCR
4.7. RNA Extraction, Reverse Transcription and PCR
4.8. Cell Fractionation
4.9. Western Blot
4.10. Tissue Immunolabelling
4.11. Cell Immunolabelling
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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mRNA | Pregnancy Period | |
---|---|---|
First Trimester (n = 16) | Term (n = 8) | |
Fold Change Average ± Standard Deviation | ||
HO1 | 1.04 ± 0.33 | 2.24 ± 0.48 *** |
SOD1 | 1.01 ± 0.16 | 3.99 ± 1.33 *** |
SOD2 | 1.15 ± 0.64 | 3.09 ± 0.85 **** |
NQO1 | 1.08 ± 0.51 | 3.53 ± 1.75 ** |
GSTA2 | 1.05 ± 0.39 | 2.42 ± 0.84 ** |
Catalase | 1.06 ± 0.37 | 6.52 ± 2.62 **** |
mRNA | Confluence | |||
---|---|---|---|---|
70% | 90% | |||
HO1 | 0.55 ± 0.06 | **** | 0.37 ± 0.05 | ****, ## |
SOD1 | 0.93 ± 0.05 | * | 0.64 ± 0.07 | ****, ### |
SOD2 | 0.99 ± 0.13 | - | 0.56 ± 0.05 | ****, ### |
NQO1 | 0.78 ± 0.11 | ** | 0.40 ± 0.07 | ****, ## |
Catalase | 1.01 ± 0.12 | - | 0.66 ± 0.07 | ****, # |
GSTA2 | 0.92 ± 0.14 | **** | 0.66 ± 0.10 | **, # |
GSS | 1.06 ± 0.14 | - | 0.77 ± 0.12 | **, # |
NRF2 | 1.02 ± 0.42 | - | 0.54 ± 0.15 | *** |
AhR | forward 5′-TAACCCAGACCAGATTCCTC-3′ |
reverse 5′-GCAAACAAAGCCAACTGAG-3′ | |
AhRR | forward 5′-CGCCTCAGTGTCAGTTACC-3′ |
reverse 5′-ACTCACGACCAGAGCAAAG-3′ | |
ARNT | forward 5′-CCCCTCCTGTAACCATTC-3′ |
reverse 5′-CTGCCCACACCAAACTG-3′ | |
β-actin | forward 5′-CTCCTTAATGTCACGCACGATTTC-3′ |
reverse 5′-ACAATGAGCTGCGTGTGGCT-3′ | |
βhCG | forward 5′-GCTACTGCCCCACCATGACC-3′ |
reverse 5′-ATGGACTCGAAGCGCACATC-3′ | |
Catalase | forward 5′-TTCATCCAGAAGAAAGCGGTCAA-3′ |
reverse 5′-TGGATGTGGCTCCCGTAGTCA-3′ | |
CYP11A1 | forward 5′-TTTTTGCCCCTGTTGGATGCA-3′ |
reverse 5′-CCCTGGCGCTCCCCAAAAAT-3′ | |
CYP19 | forward 5′-CCTGAAGCCATGCCTGCTGC-3′ |
reverse 5′-CCGATCCCCATCCACAGGAATCT-3′ | |
CYP1A1 | forward 5′-CCACAGCACAACAAGAGAC-3′ |
reverse 5′-CCATCAGGGGTGAGAAAC-3′ | |
CYP1A2 | forward 5′-TGCCAAACAGCATCATCTTG-3′ |
reverse 5′-ACAGCACAACAAGGGACACA-3′ | |
CYP1B1 | forward 5′-AACGTACCGGCCACTATCAC-3′ |
reverse 5′-CAGTGGTGGCATGAGGAATA-3′ | |
GH2 | forward 5′-CACCTTCCAACAGGGTGAAAA-3′ |
reverse 5′-GGTGGCGATAGACGTTGCT-3′ | |
GSS | forward 5′-GGAACATCCATGTGATCCGAC-3′ |
reverse 5′-GGAACATCCATGTGATCCGAC-3′ | |
GSTA2 | forward 5′-TTGGGCTCTATGGGAAGGAC-3′ |
reverse 5′-GGGAGATGTATTTGCAGCGGA-3′ | |
hPL | forward 5′-GCATGACTCCCAGACCTCCTT-3′ |
reverse 5′-TGCGGAGCAGCTCTAGATTGG-3′ | |
HO1 | forward 5′-CGTTCCTGGTCAACATCC-3′ |
reverse 5′-CTGTCGCCACCAGAAAG-3′ | |
HPRT | forward 5′-GGCGTCGTGATTAGTGATG-3′ |
reverse 5′-CAGAGGGCTACAATGTGATG-3′ | |
KRT7 | forward 5′-GGACATCGAGATCGCCACCT-3′ |
reverse 5′-ACCGCCACTGCTACTGCCA-3′ | |
NQO1 | forward 5′-GCAGACCTTGTGATATTCCAG-3′ |
reverse 5′-CCTATGAACACTCGCTCAAAC-3′ | |
NRF2 | forward 5′-TGACATACTTTGGAGGCAAG-3′ |
reverse 5′-CTAAATCAACAGGGGCTACC-3′ | |
PLGF | forward 5′-GCTCGTCAGAGGTGGAAGTGGT-3′ |
reverse 5′-CTCGCTGGGGTACTCGGACA-3 | |
RPL0 | forward 5′- AACATCTCCCCCTTCTCCT-3′ |
reverse 5′- ACTCGTTTGTACCCGTTGAT-3′ | |
RPL13 | forward 5′- AAGGTCGTGCGTCTGAAG-3′ |
reverse 5′-GAGTCCGTGGGTCTTGAG-3′ | |
SDHA | forward 5′- CCACCACTGCATCAAATTCATG-3′ |
reverse 5′-TGGGAACAAGAGGGCATCTG-3′ | |
SOD1 | forward 5′-CTGAAGGCCTGCATGGATTC-3′ |
reverse 5′-CCAAGTCTCCAACATGCCTCTC-3′ | |
SOD2 | forward 5′-GTTCAATGGTGGTGGTCATA-3′ |
reverse 5′-GTAAGTGTCCCCGTTCCTT-3′ | |
Ubiquitin C | forward 5′-CACTTGGTCCTGCGCTTGA-3′ |
reverse 5′-TTTTTTGGGAATGCAACAACTTT-3′ | |
18S | forward 5′- TCCCCCAACTTCTTAGAGG-3′ |
reverse 5′-CTTATGACCCGCACTTACTG-3′ |
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Wakx, A.; Nedder, M.; Tomkiewicz-Raulet, C.; Dalmasso, J.; Chissey, A.; Boland, S.; Vibert, F.; Degrelle, S.A.; Fournier, T.; Coumoul, X.; et al. Expression, Localization, and Activity of the Aryl Hydrocarbon Receptor in the Human Placenta. Int. J. Mol. Sci. 2018, 19, 3762. https://doi.org/10.3390/ijms19123762
Wakx A, Nedder M, Tomkiewicz-Raulet C, Dalmasso J, Chissey A, Boland S, Vibert F, Degrelle SA, Fournier T, Coumoul X, et al. Expression, Localization, and Activity of the Aryl Hydrocarbon Receptor in the Human Placenta. International Journal of Molecular Sciences. 2018; 19(12):3762. https://doi.org/10.3390/ijms19123762
Chicago/Turabian StyleWakx, Anaïs, Margaux Nedder, Céline Tomkiewicz-Raulet, Jessica Dalmasso, Audrey Chissey, Sonja Boland, Françoise Vibert, Séverine A. Degrelle, Thierry Fournier, Xavier Coumoul, and et al. 2018. "Expression, Localization, and Activity of the Aryl Hydrocarbon Receptor in the Human Placenta" International Journal of Molecular Sciences 19, no. 12: 3762. https://doi.org/10.3390/ijms19123762