Cooperative and Independent Functions of the miR-23a~27a~24-2 Cluster in Bovine Adipocyte Adipogenesis
Abstract
:1. Introduction
2. Results
2.1. Isolation and Adipogenic Differentiation of Bovine Preadipocytes
2.2. The miR-23a~27a~24-2 Cluster Represses Preadipocyte Differentiation
2.3. The miR-23a~27a~24-2 Cluster Negatively Regulates the Expression of ATP5F1, DCN, GPAM, DGAT2, G6PD, and LPL
2.4. The miR-23a~27a~24-2 Cluster Directly Targets Two Types of Genes: GPAM and DGAT2 with Pro-Adipogenic Effects and DCN, G6PD, and LPL with Anti-Adipogenic Effects
2.5. Hypothesis: There are Other Pro-Adipogenic Target Genes that Contribute to the miR-23a~27a~24-2 Cluster-Regulated Adipogenesis
2.6. FGF11 Is a Direct Target of the miR-23a~27a~24-2 Cluster
2.7. FGF11 Is a Pro-Adipogenic Mediator of Adipogenesis
3. Discussion
4. Materials and Methods
4.1. Isolation of Bovine Preadipocytes and Cell Culture
4.2. Oil Red O Staining
4.3. RNA Oligonucleotides and Transfection
4.4. Luciferase Activity Assay
4.5. ELISA Analysis of Bovine Triglyceride Content
4.6. Quantitative Real-Time-PCR
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
GPAM | glycerol-3-phosphate acyltransferase: mitochondrial |
DGAT2 | diacylglycerol O-acyltransferase 2 |
DCN | decorin |
G6PD | glucose-6-phosphate dehydrogenase |
LPL | lipoprotein lipase |
FGF11 | fibroblast growth factor 11 |
FBS | fetal bovine serum |
IBMX | 3-isobutyl-1-methylxanthine |
siRNA | short interference RNA |
NC | negative control |
3′UTR | 3′ untranslated region |
TG | triglyceride |
qRT-PCR | quantitative real time-PCR |
ANOVA | one-way analysis of variance |
ATP5F1 | ATP synthase peripheral stalk-membrane subunit b |
TAG | triacylglycerol |
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Wang, Y.; Zhang, Y.; Su, X.; Wang, H.; Yang, W.; Zan, L. Cooperative and Independent Functions of the miR-23a~27a~24-2 Cluster in Bovine Adipocyte Adipogenesis. Int. J. Mol. Sci. 2018, 19, 3957. https://doi.org/10.3390/ijms19123957
Wang Y, Zhang Y, Su X, Wang H, Yang W, Zan L. Cooperative and Independent Functions of the miR-23a~27a~24-2 Cluster in Bovine Adipocyte Adipogenesis. International Journal of Molecular Sciences. 2018; 19(12):3957. https://doi.org/10.3390/ijms19123957
Chicago/Turabian StyleWang, Yaning, Yingying Zhang, Xiaotong Su, Hongbao Wang, Wucai Yang, and Linsen Zan. 2018. "Cooperative and Independent Functions of the miR-23a~27a~24-2 Cluster in Bovine Adipocyte Adipogenesis" International Journal of Molecular Sciences 19, no. 12: 3957. https://doi.org/10.3390/ijms19123957