Glucosinolate Profiling and Expression Analysis of Glucosinolate Biosynthesis Genes Differentiate White Mold Resistant and Susceptible Cabbage Lines
Abstract
:1. Introduction
2. Results
2.1. Resistance of Different Cabbage Lines to S. sclerotiorum
2.2. Overview of Individual GSL Profiles in Cabbage Lines
2.3. Upregulation of MYB28-Bol017019, MYB34-Bol017062, ST5a-Bol026200, ST5a-Bol039395, ST5b-Bol026202 and ST5c-Bol030757 in the R Line after Inoculation
2.4. Upregulation of Transcription Factor-Related Genes and GSL Biosynthesis Genes in the S Line after Inoculation
2.5. Upregulation of Transcription-Factor-Related Genes and GSL Biosynthesis Genes in Both R and S Lines
2.6. Correlation between the Levels of Individual GSLs and the Expression Level of GSL Biosynthesis Pathway Genes Induced by S. sclerotiorum in the R and S Lines
3. Discussion
3.1. Resistance of Cabbage Lines to S. sclerotiorum
3.2. Levels of Total GSLs, GIV, and GBS Were Related to White Mold Resistance
3.3. Increased Expression of ST5b-Bol026202 and ST5c-Bol030757 Led to Increased GIV in the R Line
3.4. Increased Levels of Aliphatic GIV and Indole GBS Were Associated with White Mold Resistance
3.5. Expression of MYB28 and MYB34 Likely Induced Expression of GSL Biosynthesis Genes, Leading to Increases in GBS in the R Line
3.6. Accumulation of Indole GBS in the R Line Was Activated by Increased Expression of GSL Biosynthesis Genes
3.7. Accumulation of Aliphatic GIV with Expression of ST5b-Bol026202 and ST5c-Bol030757 and Indole GBS with ST5a-Bol026200 and ST5a-Bol039395 May Play a Role in Resistance
3.8. Association of GSL Biosynthesis Genes and Accumulation of Individual GSLs in the S Line
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Inoculum Preparation
4.3. Inoculation Technique and Disease Assessment
4.4. Leaf Sampling and Preparation for HPLC and Gene Expression Analysis
4.5. GSL Content Measurements
4.6. Primer Design for Expression Analysis of GSL Biosynthesis Genes
4.7. cDNA Synthesis and RT-qPCR Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Function of Gene Product | Genes Upregulated in R Line | Genes Upregulated in S Line | Genes Upregulated in both R and S Lines | |
---|---|---|---|---|
Higher in R Line | Higher in S Line | |||
Transcription Factor | MYB28-Bol017019 (1, 10.2); MYB34-Bol017062 (1, 3.08) | MYB28-Bol036743 (1, 5.8); MYB29-Bol008849 (1, 51.0); MYB28-Bol036286 (1, 21.6); MYB28-Bol007795 (1, 2.9) | MYB122-Bol026204 (3, 189.1) | |
Aliphatic Biosynthesis | ST5b-Bol026202 (3, 5.6); ST5c-Bol030757 (3, 68.6) | AOP2-Bo3g052110 (1, 8.4); AOP2-Bo9g006240 (1, 11.4); FMOGS-OX2-Bol010993 (1, 5.5); GSL-OH-Bol033373 (1, 65.3) | ||
Indole Biosynthesis | ST5a-Bol039395 (3, 25.3); ST5a-Bol026200 (3, 82.9) | CYP81F1-Bol017375 (1, 2.0); CYP81F1-Bol017376 (3, 7.96) | CYP81F4-Bol032712 (3, 21.1); CYP81F2-Bol026044 (3, 54.5); CYP81F2-Bol014239 (3, 871); CYP81F2-Bol012237 (3, 48.3); IGMT1-Bol007029 (3, 24.7); IGMT2-Bol007030 (3, 171) | CYP81F1-Bol028914 (3, 19.6); CYP81F4-Bol032714 (3, 12.3); IGMT1-Bol020663 (3, 652) |
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Abuyusuf, M.; Robin, A.H.K.; Lee, J.-H.; Jung, H.-J.; Kim, H.-T.; Park, J.-I.; Nou, I.-S. Glucosinolate Profiling and Expression Analysis of Glucosinolate Biosynthesis Genes Differentiate White Mold Resistant and Susceptible Cabbage Lines. Int. J. Mol. Sci. 2018, 19, 4037. https://doi.org/10.3390/ijms19124037
Abuyusuf M, Robin AHK, Lee J-H, Jung H-J, Kim H-T, Park J-I, Nou I-S. Glucosinolate Profiling and Expression Analysis of Glucosinolate Biosynthesis Genes Differentiate White Mold Resistant and Susceptible Cabbage Lines. International Journal of Molecular Sciences. 2018; 19(12):4037. https://doi.org/10.3390/ijms19124037
Chicago/Turabian StyleAbuyusuf, Md., Arif Hasan Khan Robin, Ji-Hee Lee, Hee-Jeong Jung, Hoy-Taek Kim, Jong-In Park, and Ill-Sup Nou. 2018. "Glucosinolate Profiling and Expression Analysis of Glucosinolate Biosynthesis Genes Differentiate White Mold Resistant and Susceptible Cabbage Lines" International Journal of Molecular Sciences 19, no. 12: 4037. https://doi.org/10.3390/ijms19124037