Changes in the Distribution of Cocaine- and Amphetamine-Regulated Transcript-Containing Neural Structures in the Human Colon Affected by the Neoplastic Process
Abstract
:1. Introduction
2. Results
2.1. Neurochemical Phenotype of Neurons from Studied Myenteric and Submucous Plexuses
2.2. Distribution Patterns of Nerve Fibers Containing CART in Muscle Layers of the Colonic Wall
3. Discussion
4. Materials and Methods
4.1. Patient Recruitment
4.2. Tissue Preparation
4.3. Double-Labelling Immunofluorescence
4.4. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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“Kind” of Ganglionated Plexuses | Control Tissue from Operative Margin | Cancer Affected Area | p Value |
---|---|---|---|
MP | 98.7 ± 18.0 | 73.4 ± 15.3 | 0.56 |
OSP | 12.0 ± 6.7 * | 12.5 ± 5.6 | 0.83 |
ISP | 24.7 ± 6.9 ** | 28.4 ± 9.4 | 0.68 |
Muscle Layer of Intestinal Wall | Control Tissue from Operative Margin | Cancer Affected Area |
---|---|---|
LM | 0.011 ± 0.002 | 0.014 ± 0.002 |
CM | 0.031 ± 0.004 a | 0.036 ± 0.006 b |
MM | 0.046 ± 0.014 a | 0.034 ± 0.008 |
Antibody | Species | Catalog Number | Company | Dilution |
---|---|---|---|---|
Primary antibody | ||||
CART (61–102) | rabbit | H-003-61 | Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA | 1:6000 |
PGP 9.5 | mouse | 7863-2004 | Biogenesis, Kingstone, NH, USA | 1:2100 |
Secondary antibody | ||||
Polyclonal Goat Anti-Rabbit Immunoglobulins/Biotinylated | E0432 | Dako, Glostrup, DK | 1:1000 | |
AMCA-conjugated donkey anti-mouse IgG | 715-156-151 | Jackson Immunoresearch, West Grove, PA, USA | 1:90 | |
Cy™3-conjugated streptavidin | 016-160-084 | Jackson Immunoresearch, West Grove, PA, USA | 1:9000 |
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Oponowicz, A.; Kozłowska, A.; Gonkowski, S.; Godlewski, J.; Majewski, M. Changes in the Distribution of Cocaine- and Amphetamine-Regulated Transcript-Containing Neural Structures in the Human Colon Affected by the Neoplastic Process. Int. J. Mol. Sci. 2018, 19, 414. https://doi.org/10.3390/ijms19020414
Oponowicz A, Kozłowska A, Gonkowski S, Godlewski J, Majewski M. Changes in the Distribution of Cocaine- and Amphetamine-Regulated Transcript-Containing Neural Structures in the Human Colon Affected by the Neoplastic Process. International Journal of Molecular Sciences. 2018; 19(2):414. https://doi.org/10.3390/ijms19020414
Chicago/Turabian StyleOponowicz, Agnieszka, Anna Kozłowska, Sławomir Gonkowski, Janusz Godlewski, and Mariusz Majewski. 2018. "Changes in the Distribution of Cocaine- and Amphetamine-Regulated Transcript-Containing Neural Structures in the Human Colon Affected by the Neoplastic Process" International Journal of Molecular Sciences 19, no. 2: 414. https://doi.org/10.3390/ijms19020414