Fine Mapping Identifies SmFAS Encoding an Anthocyanidin Synthase as a Putative Candidate Gene for Flower Purple Color in Solanum melongena L.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Mapping Populations
2.2. DNA Extraction and SSR Identification
2.3. Mapping Strategy and Linkage Analysis
2.4. Candidate Gene Prediction and Identification
2.5. Quantitative Real-Time RT-PCR Analysis of Candidate Genes
3. Results
3.1. Inheritance of SmFAS
3.2. Linkage Mapping of SmFAS
3.3. Candidate Genes for Purple Flower Color in Eggplant
3.4. Expression Analysis of Two ANS Genes in Various Materials and Tissues
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Population | Plant Tested | Purple:White | Mendelian Expectations | X2 | p |
---|---|---|---|---|---|
BN (purple) × SR (white) | |||||
F1 | all | all purple | 1:0 | ||
F2 | 174 | 137:37 | 3:1 | 1.295 | 0.255 |
Scaffold | CDS | Gene | Arabodopsis Homologs | Annoations | Polymorphism |
---|---|---|---|---|---|
Sme2.5_01332.1 | Sme2.5_01332.1_g00003.1 | Solyc08g079820.2.1 | AT4G11980.1 | nudix hydrolase 14 | None |
Sme2.5_01332.1_g00004.1 | Solyc08g079790.1.1 | AT1G12500.1 | sugar phosphate/phosphate translocator | None | |
Sme2.5_01332.1_g00005.1 | Solyc08g079780.1.1 | AT3G17675.1 | mavicyanin-like protein | 1 SNP | |
Sme2.5_01332.1_g00006.1 | Solyc08g079770.2.1 | AT1G12480.1 | guard cell S-type anion channel | None | |
Sme2.5_01332.1_g00007.1 | Solyc08g079760.2.1 | AT4G22860.1 | protein TPX2-like | None | |
Sme2.5_01332.1_g00008.1 | Solyc08g079750.2.1 | AT1G62960.1 | aminotransferase ACS10 | None | |
Sme2.5_01332.1_g00009.1 | Solyc08g079740.2.1 | AT1G12460.1 | LRR receptor-like serine/threonine-protein kinase | None | |
Sme2.5_01332.1_g00010.1 | Solyc12g044400.1.1 | AT3G16290.1 | inactive ATP-dependent zinc metalloprotease FTSH 12, chloroplastic | None | |
Sme2.5_01332.1_g00011.1 | Solyc08g079730.1.1 | AT4G22790.1 | protein DETOXIFICATION 56-like | None | |
Sme2.5_20506.1 | Sme2.5_20506.1_g00001.1 | Solyc08g079790.1.1 | AT1G12500.1 | sugar phosphate/phosphate translocator | None |
Sme2.5_20506.1_g00002.1 | None | ||||
Sme2.5_20506.1_g00003.1 | None | ||||
Sme2.5_25093.1 | Sme2.5_25093.1_g00001.1 | Solyc08g079820.2.1 | AT4G11980.1 | nudix hydrolase 14 | None |
Sme2.5_25093.1_g00002.1 | Solyc08g079830.2.1 | AT1G12520.1 | copper chaperone for superoxide dismutase | None | |
Sme2.5_14718.1 | Sme2.5_14718.1_g00001.1 | Solyc08g079850.1.1 | AT1G04110.1 | subtilisin-like protease SBT1.2 | 2 SNPs |
Sme2.5_05247.1 | Sme2.5_05247.1_g00002.1 | Solyc08g080010.1.1 | AT1G04110.1 | subtilisin-like protease SBT1.7 | None |
Sme2.5_05247.1_g00003.1 | Solyc08g080000.2.1 | uncharacterized | None | ||
Sme2.5_05247.1_g00004.1 | Solyc08g079980.1.1 | AT5G67360.1 | subtilisin-like protease SBT1.7 | None | |
Sme2.5_05247.1_g00005.1 | Solyc08g079970.1.1 | AT5G67360.1 | subtilisin-like protease SBT1.7 | None | |
Sme2.5_05247.1_g00006.1 | Solyc07g065420.1.1 | AT5G06990.1 | protein MIZU-KUSSEI 1 | None | |
Sme2.5_05247.1_g00008.1 | Solyc08g079870.1.1 | AT5G67360.1 | subtilisin-like protease SBT1.7 | None | |
Sme2.5_05247.1_g00009.1 | Solyc08g079870.1.1 | AT1G04110.1 | subtilisin-like protease SBT1.7 | 1 SNP | |
Sme2.5_05247.1_g000010.1 | Solyc08g079850.1.1 | AT2G05920.1 | subtilisin-like protease SBT1.7 | 3 SNPs | |
Sme2.5_05247.1_g000011.1 | subtilisin-like protease SBT1.7 | 2 SNPs | |||
Sme2.5_01638.1 | Sme2.5_01638.1_g00002.1 | Solyc08g080030.2.1 | AT1G12550.1 | glyoxylate/hydroxypyruvate reductase HPR3-like | None |
Sme2.5_01638.1_g00003.1 | Solyc08g080040.2.1 | AT4G22880.2 | Anthocyanidin Synthase (ANS) gene | None | |
Sme2.5_01638.1_g00004.1 | Solyc05g023820.2.1 | AT3G48800.1 | tuberosum myb-like protein | 2 SNPs | |
Sme2.5_01638.1_g00005.1 | Solyc08g080040.2.1 | AT4G22880.2 | Anthocyanidin Synthase (ANS) gene | 4 SNPs and 1 InDel | |
Sme2.5_01638.1_g00006.1 | Solyc08g080050.2.1 | AT4G22890.2 | PGR5-like protein | None |
Eggplant Cultivar | Color of Flower | Color of Peel |
---|---|---|
6 | purple | green |
9 | purple | white |
164A | purple | white |
247 | purple | white |
264 | purple | green |
267 | purple | green |
282 | purple | green |
285 | purple | white |
290 | purple | green |
295 | purple | white |
296 | purple | white |
297 | purple | white |
BN | purple | purple |
221 | white | green |
299 | white | green |
SR | white | green |
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Chen, M.; Xu, M.; Xiao, Y.; Cui, D.; Qin, Y.; Wu, J.; Wang, W.; Wang, G. Fine Mapping Identifies SmFAS Encoding an Anthocyanidin Synthase as a Putative Candidate Gene for Flower Purple Color in Solanum melongena L. Int. J. Mol. Sci. 2018, 19, 789. https://doi.org/10.3390/ijms19030789
Chen M, Xu M, Xiao Y, Cui D, Qin Y, Wu J, Wang W, Wang G. Fine Mapping Identifies SmFAS Encoding an Anthocyanidin Synthase as a Putative Candidate Gene for Flower Purple Color in Solanum melongena L. International Journal of Molecular Sciences. 2018; 19(3):789. https://doi.org/10.3390/ijms19030789
Chicago/Turabian StyleChen, Mengqiang, Mengyun Xu, Yao Xiao, Dandan Cui, Yongqiang Qin, Jiaqi Wu, Wenyi Wang, and Guoping Wang. 2018. "Fine Mapping Identifies SmFAS Encoding an Anthocyanidin Synthase as a Putative Candidate Gene for Flower Purple Color in Solanum melongena L." International Journal of Molecular Sciences 19, no. 3: 789. https://doi.org/10.3390/ijms19030789