Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. The Effect of Formononetin in Cisplatin-Induced Cell Death in LLC-PK1 Cells
2.2. Formononetin Did Not Alter the Anticancer Effect of Cisplatin on Three Different Cervical Cancer Cell Lines, Including HeLa, SiHa, and CaSKi
2.3. Formononetin Prevented Intracellular Reactive Oxygen Speiceses (ROS) Accumulation
2.4. Formononetin Prevented Cisplatin-Induced Apoptotic LLC-PK1 Cell Death
2.5. Formononetin Inhibited Cisplatin-Meidiated Phosphorylation of JNK, Cleavage of Caspase-8 and Caspase-3, and the Ratio of Bax to Bcl-2
3. Materials and Methods
3.1. Chemicals and Reagents
3.2. Cell Cultures
3.3. Cell Viability Assay
3.4. Determination of Intracellular Reactive Oxygen Species
3.5. Nuclear Staining with Hoechst 33342
3.6. Quantification of Apoptotic Cell Death
3.7. Western Blotting Analysis
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, H.; Lee, D.; Kang, K.S.; Song, J.H.; Choi, Y.-K. Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells. Int. J. Mol. Sci. 2018, 19, 813. https://doi.org/10.3390/ijms19030813
Lee H, Lee D, Kang KS, Song JH, Choi Y-K. Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells. International Journal of Molecular Sciences. 2018; 19(3):813. https://doi.org/10.3390/ijms19030813
Chicago/Turabian StyleLee, Haesol, Dahae Lee, Ki Sung Kang, Ji Hoon Song, and You-Kyoung Choi. 2018. "Inhibition of Intracellular ROS Accumulation by Formononetin Attenuates Cisplatin-Mediated Apoptosis in LLC-PK1 Cells" International Journal of Molecular Sciences 19, no. 3: 813. https://doi.org/10.3390/ijms19030813