HER2-Targeted Multifunctional Silica Nanoparticles Specifically Enhance the Radiosensitivity of HER2-Overexpressing Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. PCSN Probes
2.2. Inhibition of Cell Growth by PCSNs
2.3. Combination of Radiation Therapy and PCSN Probes
3. Discussion
4. Materials and Methods
4.1. PCSN Probes
4.2. Cell Culture
4.3. Internalization of PCSN Probes by HER2-Overexpressing Cells
4.4. Transmission Electron Microscopy (TEM)
4.5. Evaluation of Cell Viability Using Several Concentrations of PAMAM Silica Nanoparticles
4.6. The Combination of PCSN Probes and Irradiation
4.7. Observation of Cell Viability
4.8. TUNEL Assay
4.9. FLICA Assay
4.10. Fluorescent Staining of Cell Organelles
4.11. Data Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yamaguchi, H.; Hayama, K.; Sasagawa, I.; Okada, Y.; Kawase, T.; Tsubokawa, N.; Tsuchimochi, M. HER2-Targeted Multifunctional Silica Nanoparticles Specifically Enhance the Radiosensitivity of HER2-Overexpressing Breast Cancer Cells. Int. J. Mol. Sci. 2018, 19, 908. https://doi.org/10.3390/ijms19030908
Yamaguchi H, Hayama K, Sasagawa I, Okada Y, Kawase T, Tsubokawa N, Tsuchimochi M. HER2-Targeted Multifunctional Silica Nanoparticles Specifically Enhance the Radiosensitivity of HER2-Overexpressing Breast Cancer Cells. International Journal of Molecular Sciences. 2018; 19(3):908. https://doi.org/10.3390/ijms19030908
Chicago/Turabian StyleYamaguchi, Haruka, Kazuhide Hayama, Ichiro Sasagawa, Yasuo Okada, Tomoyuki Kawase, Norio Tsubokawa, and Makoto Tsuchimochi. 2018. "HER2-Targeted Multifunctional Silica Nanoparticles Specifically Enhance the Radiosensitivity of HER2-Overexpressing Breast Cancer Cells" International Journal of Molecular Sciences 19, no. 3: 908. https://doi.org/10.3390/ijms19030908