Nitrate Reductases Are Relocalized to the Nucleus by AtSIZ1 and Their Levels Are Negatively Regulated by COP1 and Ammonium
Abstract
:1. Introduction
2. Results
2.1. NR Proteins and AtSIZ1 Localize to the Nucleus
2.2. Nuclear Localization of NR Proteins Is Not Affected by Nitrate
2.3. NIA1 and NIA2 Are Not Detected in Ammonium-Treated Cells
2.4. NIA1 and NIA2 Levels Are Regulated by Nitrate and Ammonium Sources
2.5. NR Proteins Accumulate in cop1-4 and DN-COP1-Overexpressing Plants
3. Discussion
4. Materials and Methods
4.1. Plant Material and Culture Conditions
4.2. Subcellular Localization
4.3. Quantitative RT-PCR
4.4. Examination of NR Protein Levels
4.5. Cell-Free Degradation Assay
4.6. Yeast Two-Hybrid Experiments
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Leaf | Root | ||||||||
---|---|---|---|---|---|---|---|---|---|
None | K2SO4 | KNO3 | (NH4)2SO4 | None | K2SO4 | KNO3 | (NH4)2SO4 | ||
NIA1 | 1.00 ± 0.18 | 1.02 ± 0.15 | 3.33 ± 0.27 | 2.23 ± 0.24 | 1.00 ± 0.12 | 1.10 ± 0.21 | 1.62 ± 0.44 | 1.56 ± 0.07 | |
NIA2 | 1.00 ± 0.19 | 1.01 ± 0.13 | 2.00 ± 0.11 | 1.36 ± 0.12 | 1.00 ± 0.13 | 0.98 ± 0.14 | 1.59 ± 0.36 | 1.34 ± 0.08 |
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Kim, J.Y.; Park, B.S.; Park, S.W.; Lee, H.Y.; Song, J.T.; Seo, H.S. Nitrate Reductases Are Relocalized to the Nucleus by AtSIZ1 and Their Levels Are Negatively Regulated by COP1 and Ammonium. Int. J. Mol. Sci. 2018, 19, 1202. https://doi.org/10.3390/ijms19041202
Kim JY, Park BS, Park SW, Lee HY, Song JT, Seo HS. Nitrate Reductases Are Relocalized to the Nucleus by AtSIZ1 and Their Levels Are Negatively Regulated by COP1 and Ammonium. International Journal of Molecular Sciences. 2018; 19(4):1202. https://doi.org/10.3390/ijms19041202
Chicago/Turabian StyleKim, Joo Yong, Bong Soo Park, Sang Woo Park, Han Yong Lee, Jong Tae Song, and Hak Soo Seo. 2018. "Nitrate Reductases Are Relocalized to the Nucleus by AtSIZ1 and Their Levels Are Negatively Regulated by COP1 and Ammonium" International Journal of Molecular Sciences 19, no. 4: 1202. https://doi.org/10.3390/ijms19041202