Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola
Abstract
:1. Introduction
2. Results
2.1. PRR Expression in Vitis amurensis ‘Shuanghong’ Infected with Incompatible and Compatible Strains of Plasmopara viticola
2.2. Characterization and Phylogenetic Analysis of VaHAESA
2.3. Subcellular Location of VaHAESA in Nicotiana benthamiana
2.4. Expression of VaHAESA Promoted Resistance against Plasmopara viticola in Grapevine
2.5. Measurements of H2O2, NO, and Callose in Vitis vinifera Transiently Expressing VaHAESA
2.6. Identification and Analysis of Disease Resistance in Transgenic Arabidopsis thaliana
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Plasmopara viticola Strains, and Pathogen Infection
4.2. qRT-PCR
4.3. Cloning, Sequencing, and Phylogenetic Characterization of VaHAESA
4.4. Agrobacterium-Mediated Transient Expression in Plants
4.5. Analysis of the Subcellular Localization of VaHAESA and Its Effect on Pathogen Infection
4.6. Analysis of H2O2 and NO Levels in Transgenic Vitis vinifera Expressing VaHAESA
4.7. Screening and Identification of Transgenic Arabidopsis thaliana
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Liu, S.; Zhang, C.; Chao, N.; Lu, J.; Zhang, Y. Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola. Int. J. Mol. Sci. 2018, 19, 1204. https://doi.org/10.3390/ijms19041204
Liu S, Zhang C, Chao N, Lu J, Zhang Y. Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola. International Journal of Molecular Sciences. 2018; 19(4):1204. https://doi.org/10.3390/ijms19041204
Chicago/Turabian StyleLiu, Shaoli, Chi Zhang, Nan Chao, Jiang Lu, and Yali Zhang. 2018. "Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola" International Journal of Molecular Sciences 19, no. 4: 1204. https://doi.org/10.3390/ijms19041204