Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice
Abstract
:1. Introduction
2. Results
2.1. Identification and Isolation of a T-DNA Inserted Allele in the Rice Osfuct Gene
2.2. Osfuct Mutant Significantly Affects Anther Development
2.3. Rescue of Mutant Phenotype by Wild-Type Osfuct Transgene
2.4. Effect of Osfuct Mutation on Pollen Development
2.5. The Glycosylation Pattern Is Altered in the Osfuct Mutant
2.6. Osfuct Mutant Exhibits Sensitivity to Salt Stress
2.7. Transcriptomic Profiling of the Osfuct Mutant
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions for Mutant Screening
4.2. Genotyping and Phylogenic Analysis of the Osfuct Mutant
4.3. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Quantitative Real-Time PCR (qPCR) Analysis
4.4. Phenotypic Analyses of the Osfuct Mutant
4.5. Assessment of Reproductive Development of the Osfuct Mutant
4.6. Vector Construction for Complementation Analysis of Osfuct Disrupted Mutant
4.7. N-Glycan Profiling of the Osfuct Mutant
4.8. Salt Tolerance Assay of the Osfuct Mutant
4.9. Transcriptome Analyses of the Osfuct Mutant
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
OsFucT | α1,3-fucosyltransferase |
MALDI-TOF | Matrix-assisted laser desorption/ionization time-of-flight |
ORF | Open reading frame |
HM | Homozygote |
HT | Heterozygote |
SEM | Scanning electron microscopy |
GEO | Gene Expression Omnibus |
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Floral Parts | Dimensions (mm) | DJ | HM | HT | R12 |
---|---|---|---|---|---|
Mature flower | Length (mm) | 6.78 ± 0.28 | 6.12 ± 0.20 * | 6.29 ± 0.32 * | 6.45 ± 0.29 |
Width (mm) | 2.65 ± 0.22 | 2.61 ± 0.25 | 2.66 ± 0.34 | 2.62 ± 0.22 | |
Anther | Length (mm) | 2.03 ± 0.14 | 1.35 ± 0.27 * | 1.85 ± 0.11 * | 1.81 ± 0.07 * |
Width (mm) | 0.38 ± 0.03 | 0.35 ± 0.03 | 0.39 ± 0.04 | 0.38 ± 0.01 | |
Pistil | Length of pistil (mm) | 1.74 ± 0.15 | 1.47 ± 0.22 * | 1.79 ± 0.20 | 1.77 ± 0.15 |
Width of the ovule (mm) | 0.38 ± 0.02 | 0.39 ± 0.02 | 0.40 ± 0.03 | 0.38 ± 0.01 |
SN | Gene ID | Gene Location | DJ vs. HM (log2 Fold Change) | Gene Descriptions |
---|---|---|---|---|
1 | Os03g0299700 | 10495846..10498081 | 46.75 | Hypothetical protein |
2 | - | - | 32.96 | Hygromycin * |
3 | Os03g0629800 | 24806433..24807496 | 24.06 | Hypothetical protein |
4 | Os01g0965300 | 42554248..42554866 | 14.77 | Hypothetical protein |
5 | Os03g0105500 | 338364..338744 | 8.03 | Hypothetical protein |
6 | Os02g0269650 | 9689236..9689758 | 5.41 | Hypothetical protein |
7 | Os10g0340100 | 9458993..9461016 | 5.14 | Hypothetical protein |
8 | Os04g0112100 | 692745..695801 | 4.55 | Putative disease resistance protein RGA4 |
9 | Os09g0356800 | 11483610..11506480 | 4.38 | LRR receptor-like serine/threonine-protein kinase |
10 | Os04g0397800 | 19645507..19646962 | 4.32 | Hypothetical protein |
11 | Os12g0543800 | 21881810..21883709 | 4.29 | Hypothetical protein |
12 | Os07g0511400 | 20194023..20194735 | 3.48 | Hypothetical protein |
13 | Os10g0142600 | 2591399..2596634 | 3.20 | Putative wall-associated protein kinase |
14 | Os10g0142700 | 2573722..2575920 | 3.05 | Putative wall-associated protein kinase (non-protein coding RNA) |
15 | Os08g0193600 | 5457436..5458183 | 2.72 | F-box protein-like |
16 | Os01g0204900 | 5756471..5757587 | 2.44 | Hypothetical protein |
17 | Os01g0605500 | 23850865..23854747 | −5.39 | Kinesin-like protein KIF19 |
18 | Os04g0116800 | 984402..985981 | −5.90 | Hypothetical protein |
19 | Os04g0103800 | 250445..251491 | −8.59 | WASH complex subunit CCDC53 homolog |
20 | Os08g0472600 | 23273330..23281518 | −42.45 | Glycoprotein 3-alpha-l-fucosyltransferase A |
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Sim, J.-S.; Kesawat, M.S.; Kumar, M.; Kim, S.-Y.; Mani, V.; Subramanian, P.; Park, S.; Lee, C.-M.; Kim, S.-R.; Hahn, B.-S. Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice. Int. J. Mol. Sci. 2018, 19, 1225. https://doi.org/10.3390/ijms19041225
Sim J-S, Kesawat MS, Kumar M, Kim S-Y, Mani V, Subramanian P, Park S, Lee C-M, Kim S-R, Hahn B-S. Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice. International Journal of Molecular Sciences. 2018; 19(4):1225. https://doi.org/10.3390/ijms19041225
Chicago/Turabian StyleSim, Joon-Soo, Mahipal Singh Kesawat, Manu Kumar, Su-Yeon Kim, Vimalraj Mani, Parthiban Subramanian, Soyoung Park, Chang-Muk Lee, Seong-Ryong Kim, and Bum-Soo Hahn. 2018. "Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice" International Journal of Molecular Sciences 19, no. 4: 1225. https://doi.org/10.3390/ijms19041225