An SCFFBXO28 E3 Ligase Protects Pancreatic β-Cells from Apoptosis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Loss of FBXO28 Induces β-Cell Apoptosis
2.2. Overexpression of FBXO28 Protects β-Cells from Apoptosis
2.3. FBXO28 Does Not Regulate β-Cell Function
2.4. FBXO28 Overexpression Does Not Improve β-Cell Function in T2D Human Islets
3. Materials and Methods
3.1. Islet Isolation, Cell Culture, and Treatment
3.2. Transfections
3.3. Adenoviral Infection
3.4. Western Blot Analysis
3.5. Glucose-Stimulated Insulin Secretion
3.6. RNA Extraction and RT-PCR Analysis
3.7. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gorrepati, K.D.D.; He, W.; Lupse, B.; Yuan, T.; Maedler, K.; Ardestani, A. An SCFFBXO28 E3 Ligase Protects Pancreatic β-Cells from Apoptosis. Int. J. Mol. Sci. 2018, 19, 975. https://doi.org/10.3390/ijms19040975
Gorrepati KDD, He W, Lupse B, Yuan T, Maedler K, Ardestani A. An SCFFBXO28 E3 Ligase Protects Pancreatic β-Cells from Apoptosis. International Journal of Molecular Sciences. 2018; 19(4):975. https://doi.org/10.3390/ijms19040975
Chicago/Turabian StyleGorrepati, Kanaka Durga Devi, Wei He, Blaz Lupse, Ting Yuan, Kathrin Maedler, and Amin Ardestani. 2018. "An SCFFBXO28 E3 Ligase Protects Pancreatic β-Cells from Apoptosis" International Journal of Molecular Sciences 19, no. 4: 975. https://doi.org/10.3390/ijms19040975