Turnover of Glycerolipid Metabolite Pool and Seed Viability
Abstract
:1. Introduction
2. Results
2.1. The Viability of Seeds Gradually Decreased as the Number of Hydration–Dehydration Cycles Increased
2.2. The Total Membrane Lipid Contents of A. thaliana Seeds Changed Significantly through the Hydration–Dehydration Cycles
2.3. Changes in Lipid Classes during the Hydration–Dehydration Cycles
2.4. Downward Trends in MGDG and DGDG Contents during the Hydration–Dehydration Cycles
2.5. DAG and PA Mediated Glycerolipid Changes during the Hydration–Dehydration Cycles
2.6. Membrane Lipids Became More Unsaturated during Dehydration
2.7. The PS ACL Increased during the Hydration–Dehydration Cycles
2.8. The Profiling of Lyso-Phospholipids during Continuous Hydration–Dehydration Cycles
3. Discussion
4. Materials and Methods
4.1. Seed Materials
4.2. Hydration–Dehydration Cycles and Sampling Scheme
4.3. Seed Germination
4.4. Lipid Extraction, Electrospray Ionization Tandem Mass Spectrometry Analysis, and Data Processing
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
PG | phosphatidylglycerol |
PC | phosphatidylcholine |
PE | phosphatidylethanolamine |
PI | phosphatidylinositol |
PS | phosphatidylserine |
PA | phosphatidic acid |
MGDG | monogalactosyldiacylglycerol |
DGDG | digalactosyldiacylglycerol |
DAG | diacylglycerol |
ER | endoplasmic reticulum |
DBI | double bond index |
ACL | acyl chain lengths |
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Double Bond Index (DBI) | ||||
---|---|---|---|---|
C | 3H | 3D | 4H | 6D |
3.05 ± 0.02 c | 3.05 ± 0.02 c | 3.13 ± 0.03 b | 3.02 ± 0.04 c | 3.31 ± 0.02 a |
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Hu, X.-L.; Yu, X.-M.; Chen, H.-Y.; Li, W.-Q. Turnover of Glycerolipid Metabolite Pool and Seed Viability. Int. J. Mol. Sci. 2018, 19, 1417. https://doi.org/10.3390/ijms19051417
Hu X-L, Yu X-M, Chen H-Y, Li W-Q. Turnover of Glycerolipid Metabolite Pool and Seed Viability. International Journal of Molecular Sciences. 2018; 19(5):1417. https://doi.org/10.3390/ijms19051417
Chicago/Turabian StyleHu, Xiao-Long, Xiao-Mei Yu, Hong-Ying Chen, and Wei-Qi Li. 2018. "Turnover of Glycerolipid Metabolite Pool and Seed Viability" International Journal of Molecular Sciences 19, no. 5: 1417. https://doi.org/10.3390/ijms19051417