Lambertianic Acid Sensitizes Non-Small Cell Lung Cancers to TRAIL-Induced Apoptosis via Inhibition of XIAP/NF-κB and Activation of Caspases and Death Receptor 4
Abstract
:1. Introduction
2. Results
2.1. Combined Treatment of LA and TRAIL Enhanced Cytotoxicity in A549 and H1299 Non-Small Cell Lung Cancer Cells
2.2. Combined Treatment of LA and TRAIL Significantly Increased the Sub-G1 Population and Also Increased the Cleavage of PARP and Caspase8/9/3 in A549 and H1299 Non-Small Cell Lung Cancer Cells
2.3. Combined Treatment of LA and TRAIL Regulated Antiapoptotic and Proapoptotic Proteins in A549 and H1299 Non-Small Cell Lung Cancer Cells
2.4. Combined Treatment of LA and TRAIL Upregulated the Expression of DR4 and Inhibited the Expression of p-NF-κB, p-IκB, and FLIP in A549 and H1299 Non-Small Cell Lung Cancer Cells
2.5. Combined Treatment of LA and TRAIL Disrupted Binding of XIAP with Caspase3 and NF-κB in A549 Non-Small Cell Lung Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Lambertianic Acid Isolation
4.2. Cell Culture
4.3. Cytotoxicity Assay
4.4. Crystal Violet Assay
4.5. Cell Cycle Analysis
4.6. Western Blotting
4.7. Co-Immunoprecipitation
4.8. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
TRAIL | TNF-related apoptosis-inducing ligand |
PARP | Poly (ADP-ribose) polymerase |
Caspase | Cysteine aspartyl-specific protease |
Bcl-2 | B-cell lymphoma 2 |
XIAP | X-linked inhibitor of apoptosis protein |
DR5 | Death receptor5 |
NF-κB | Nuclear factor kappa-light-chain-enhancer of activated B cells |
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Ahn, D.S.; Lee, H.J.; Hwang, J.; Han, H.; Kim, B.; Shim, B.; Kim, S.-H. Lambertianic Acid Sensitizes Non-Small Cell Lung Cancers to TRAIL-Induced Apoptosis via Inhibition of XIAP/NF-κB and Activation of Caspases and Death Receptor 4. Int. J. Mol. Sci. 2018, 19, 1476. https://doi.org/10.3390/ijms19051476
Ahn DS, Lee HJ, Hwang J, Han H, Kim B, Shim B, Kim S-H. Lambertianic Acid Sensitizes Non-Small Cell Lung Cancers to TRAIL-Induced Apoptosis via Inhibition of XIAP/NF-κB and Activation of Caspases and Death Receptor 4. International Journal of Molecular Sciences. 2018; 19(5):1476. https://doi.org/10.3390/ijms19051476
Chicago/Turabian StyleAhn, Deok Soo, Hyo Jung Lee, Jisung Hwang, Hyukgyu Han, Bonglee Kim, BumSang Shim, and Sung-Hoon Kim. 2018. "Lambertianic Acid Sensitizes Non-Small Cell Lung Cancers to TRAIL-Induced Apoptosis via Inhibition of XIAP/NF-κB and Activation of Caspases and Death Receptor 4" International Journal of Molecular Sciences 19, no. 5: 1476. https://doi.org/10.3390/ijms19051476