Protective Effects of 2-Amino-5,6-dihydro-4H-1,3-thiazine and Its Derivative against Radiation-Induced Hematopoietic and Intestinal Injury in Mice
Abstract
:1. Introduction
2. Results
2.1. 2-ADT and 2-AADT Improved the Survival of Mice Exposed to a Lethal Dose of Irradiation
2.2. 2-ADT and 2-AADT Promoted Recovery of Hematopoietic System
2.3. 2-ADT and 2-AADT Enhanced the Activity of Various Antioxidant Enzymes and Reduced Lipid Peroxidation
2.4. 2-ADT and 2-AADT Attenuated Radiation-Induced Elevation of NO in Plasma
2.5. 2-ADT and 2-AADT Alleviated Radiation-Induced DNA Damage to Peripheral Blood Lymphocytes
2.6. 2-ADT and 2-AADT Ameliorated Small Intestinal Injury in Mice after Abdominal Irradiation
2.7. 2-ADT and 2-AADT Promoted the Proliferation of Vill Enterocytes, Paneth Cells and Ki67 Transient Amplifying Cells in the Small Intestine of Mice after Abdominal Irradiation
2.8. 2-ADT and 2-AADT Attenuated Radiation-Induced Nitrotyrosine Expression in the Small Intestine after Abdominal Irradiation
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Mice and Irradiation
4.3. Survival Study
4.4. Determination of the Effect of 2-ADT and 2-AADT on Radiation Induced Hematopoietic System Injury
4.4.1. Hematological Parameters
4.4.2. Colony Forming Assay
4.4.3. Bone Marrow Nucleated Cells and DNA Content
4.5. Biochemical Determination
4.6. Plasma NOx Detection
4.7. Comet Assay
4.8. Determination of the Effects of 2-ADT and 2-AADT on Intestinal Injury in Irradiated Mice
4.8.1. Histopathology
4.8.2. Immunohistochemistry
4.9. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Li, Y.; Kong, S.; Yang, F.; Xu, W. Protective Effects of 2-Amino-5,6-dihydro-4H-1,3-thiazine and Its Derivative against Radiation-Induced Hematopoietic and Intestinal Injury in Mice. Int. J. Mol. Sci. 2018, 19, 1530. https://doi.org/10.3390/ijms19051530
Li Y, Kong S, Yang F, Xu W. Protective Effects of 2-Amino-5,6-dihydro-4H-1,3-thiazine and Its Derivative against Radiation-Induced Hematopoietic and Intestinal Injury in Mice. International Journal of Molecular Sciences. 2018; 19(5):1530. https://doi.org/10.3390/ijms19051530
Chicago/Turabian StyleLi, Yuanyuan, Shaofan Kong, Fujun Yang, and Wenqing Xu. 2018. "Protective Effects of 2-Amino-5,6-dihydro-4H-1,3-thiazine and Its Derivative against Radiation-Induced Hematopoietic and Intestinal Injury in Mice" International Journal of Molecular Sciences 19, no. 5: 1530. https://doi.org/10.3390/ijms19051530