A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Surgical Procedure to Harvest Adipose Tissue
4.2. Isolation, Expansion and Viability of ASCs
4.3. Growth Curve
4.4. Flow Cytometry Analysis and Phenotype Characterization
4.5. Real-Time PCR
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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De Francesco, F.; Mannucci, S.; Conti, G.; Dai Prè, E.; Sbarbati, A.; Riccio, M. A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results. Int. J. Mol. Sci. 2018, 19, 2061. https://doi.org/10.3390/ijms19072061
De Francesco F, Mannucci S, Conti G, Dai Prè E, Sbarbati A, Riccio M. A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results. International Journal of Molecular Sciences. 2018; 19(7):2061. https://doi.org/10.3390/ijms19072061
Chicago/Turabian StyleDe Francesco, Francesco, Silvia Mannucci, Giamaica Conti, Elena Dai Prè, Andrea Sbarbati, and Michele Riccio. 2018. "A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results" International Journal of Molecular Sciences 19, no. 7: 2061. https://doi.org/10.3390/ijms19072061