Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars
Abstract
:1. Introduction
2. Results
2.1. Manufacture of PDMS/GO Membrane
2.2. Comparison of the Characteristics of Pure PDMS and Other GO-Modified PDMS Elastomers
2.3. Introduction of Hexagonal Micropillars onto the GO-Enriched and GO-Coated PDMS Scaffolds
2.4. Physical Characteristics of the GO-Enriched and GO-Coated PDMS Scaffold with Hexagonal Micropillars
2.5. Biocompatibility of PDMS/GO Scaffold with hiPSC-RPE Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals and Materials
4.2. Fabrication of PDMS/GO Membrane
4.3. PDMS Scaffold Surface Modification
4.4. Atomic Force Microscopy
4.5. Contact Angle Measurement
4.6. FTIR Characterization
4.7. Mechanical Properties Measurement with Nanoindentation
4.8. Membrane Resistance Analysis (Ion Permeability Test)
4.9. Human Induced Pluripotent Stem Cells (hiPSCs) Cultivation and Differentiation to RPEs
4.10. Immunofluorescence Staining
4.11. Quantitative RT-PCR
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PDMS | polydimethylsiloxane |
GO | graphene oxide |
hiPSC | human induced pluripotent stem cell |
hiPSC-RPEs | hiPSC-differentiated retinal pigment epithelial cells |
ZO-1 | zonula occludens-1 |
PEDF | pigment epithelium-derived factor |
RPE65 | retinal pigmented epithelium-specific protein with molecular mass 65 kDa |
BDNF | brain-derived neurotrophic factor |
CCD | charge-coupled device |
NEAA | nonessential amino acids |
DKK1 | recombinant Dickkopf-related protein 1 |
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Lin, Y.-Y.; Chien, Y.; Chuang, J.-H.; Chang, C.-C.; Yang, Y.-P.; Lai, Y.-H.; Lo, W.-L.; Chien, K.-H.; Huo, T.-I.; Wang, C.-Y. Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars. Int. J. Mol. Sci. 2018, 19, 2517. https://doi.org/10.3390/ijms19092517
Lin Y-Y, Chien Y, Chuang J-H, Chang C-C, Yang Y-P, Lai Y-H, Lo W-L, Chien K-H, Huo T-I, Wang C-Y. Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars. International Journal of Molecular Sciences. 2018; 19(9):2517. https://doi.org/10.3390/ijms19092517
Chicago/Turabian StyleLin, Yi-Ying, Yueh Chien, Jen-Hua Chuang, Chia-Ching Chang, Yi-Ping Yang, Ying-Hsiu Lai, Wen-Liang Lo, Ke-Hung Chien, Teh-Ia Huo, and Chien-Ying Wang. 2018. "Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars" International Journal of Molecular Sciences 19, no. 9: 2517. https://doi.org/10.3390/ijms19092517