CCAR2/DBC1 and Hsp60 Positively Regulate Expression of Survivin in Neuroblastoma Cells
Abstract
:1. Introduction
2. Results
2.1. The CCAR2-Hsp60 Complex Binds the Anti-Apoptotic Protein Survivin
2.2. Both CCAR2 and Hsp60 are Required for Expression of Survivin
2.3. CCAR2 and Hsp60 Regulate Expression of Survivin mRNA
2.4. Expression of CCAR2 and Hsp60 in Patients with Neuroblastoma Correlate Positively with that of Survivin
2.5. Expression of CCAR2, Hsp60, and Survivin Shows a Negative Correlation with Survival of Neuroblastoma Patients
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Small Interfering RNA (siRNA) Transfection
4.3. Immunoprecipitation and Western blotting
4.4. Subcellular Fractionation
4.5. Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
4.6. Analysis of Correlation between Gene Expressions
4.7. Analysis of the Association between Gene Expression and Survival of Neuroblastoma Patients
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CCAR2 | Cell cycle and apoptosis regulator 2 |
Hsp60 | Heat shock protein 60 |
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Kim, W.; Ryu, J.; Kim, J.-E. CCAR2/DBC1 and Hsp60 Positively Regulate Expression of Survivin in Neuroblastoma Cells. Int. J. Mol. Sci. 2019, 20, 131. https://doi.org/10.3390/ijms20010131
Kim W, Ryu J, Kim J-E. CCAR2/DBC1 and Hsp60 Positively Regulate Expression of Survivin in Neuroblastoma Cells. International Journal of Molecular Sciences. 2019; 20(1):131. https://doi.org/10.3390/ijms20010131
Chicago/Turabian StyleKim, Wootae, Jaewook Ryu, and Ja-Eun Kim. 2019. "CCAR2/DBC1 and Hsp60 Positively Regulate Expression of Survivin in Neuroblastoma Cells" International Journal of Molecular Sciences 20, no. 1: 131. https://doi.org/10.3390/ijms20010131