Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
Abstract
:1. Introduction
2. Results
2.1. Content Characterization of Plasma FXIII Concentrate Reveals Co-Presence of Complement Factor H and Alpha-2-Macroglobulin Along with FXIII Complex
2.2. No Significant Effect of CFH on FXIII-Aa Activation Observed in the FXIIIa Generation Assay
2.3. In a FXIII Deficient Background, FXIII-B Pulls Down Fibrinogen, Few Complement Proteins, and Alpha-2-Macroglobulin
3. Discussion
4. Materials and Methods
4.1. Coagulation Factor XIII Complex and FXIII Subunits
4.2. Separation of the FXIII Plasma Concentrate, FibrogamminP into its Constituents
4.3. Mass Spectrometric Analysis
4.4. FXIIIa Generation Assay
4.5. Isolation and Verification of Plasma Sub-Proteome Interacting with FXIII-B by Immunoaffinity Based Pull-Down Assays
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
FXIII-DP | Factor XIII deficient plasma |
CFH | Complement Factor H |
FXIIIa | Activated FXIII-A |
SP | Standard Plasma |
MS | Mass spectrometry |
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Singh, S.; Akhter, M.S.; Dodt, J.; Volkers, P.; Reuter, A.; Reinhart, C.; Krettler, C.; Oldenburg, J.; Biswas, A. Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder. Int. J. Mol. Sci. 2019, 20, 2682. https://doi.org/10.3390/ijms20112682
Singh S, Akhter MS, Dodt J, Volkers P, Reuter A, Reinhart C, Krettler C, Oldenburg J, Biswas A. Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder. International Journal of Molecular Sciences. 2019; 20(11):2682. https://doi.org/10.3390/ijms20112682
Chicago/Turabian StyleSingh, Sneha, Mohammad Suhail Akhter, Johannes Dodt, Peter Volkers, Andreas Reuter, Christoph Reinhart, Christoph Krettler, Johannes Oldenburg, and Arijit Biswas. 2019. "Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder" International Journal of Molecular Sciences 20, no. 11: 2682. https://doi.org/10.3390/ijms20112682