HNF4α and CDX2 Regulate Intestinal YAP1 Promoter Activity
Abstract
:1. Introduction
2. Results
2.1. The Chromatin Landscape of the YAP1 Gene
2.1.1. YAP1 Promoter Activity
2.1.2. CDX2 and HNF4α Binding Sites in the YAP1 Intragenic Enhancer
2.1.3. CDX2 Activation of YAP1 Protein Expression
3. Discussion
4. Materials and Methods
4.1. Culture of Human Cell Lines
4.2. Bioinformatic Analysis of the YAP1 Gene
4.3. Construction of Luciferase Reporter Constructs
4.4. Construction of Reporter Constructs with Mutated Binding Sites
4.5. Transfection of Cell Lines
4.6. Measuring Luciferase Activity of Reporter Constructs
4.7. Chromatin Immunoprecipitation Assay and Quantitative PCR
4.8. Electrophoretic Mobility Shift Assay
4.9. Western Blotting
4.10. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
YAP1 | Yes-associated protein 1 |
WWTR1 | WW domain containing transcription regulator 1 |
TEAD | TEA domain transcription factor |
ERG | Transcriptional regulator ERG |
TEAD4 HA | TEA domain transcription factor 4 Hemagglutinin |
PCOS GAPDH | Polycystic ovary syndrome Glyceraldehyde-3-phosphate dehydrogenase |
GATA4/5/6 | GATA-binding proteins 4/5/6 |
HNF1A/B | HNF1 homeobox proteins |
TCF7L2 | Transcription factor 7 like 2 |
HNF4α | Hepatocyte nuclear factor 4 alpha |
CDX2 | Caudal type homeobox 2 |
EMSA | Electrophoretic mobility shift assay |
HA | Hemagglutinin |
PEI | Polyethyleneimine |
AMPS | Ammonium persulfate |
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Larsen, S.; Davidsen, J.; Dahlgaard, K.; Pedersen, O.B.; Troelsen, J.T. HNF4α and CDX2 Regulate Intestinal YAP1 Promoter Activity. Int. J. Mol. Sci. 2019, 20, 2981. https://doi.org/10.3390/ijms20122981
Larsen S, Davidsen J, Dahlgaard K, Pedersen OB, Troelsen JT. HNF4α and CDX2 Regulate Intestinal YAP1 Promoter Activity. International Journal of Molecular Sciences. 2019; 20(12):2981. https://doi.org/10.3390/ijms20122981
Chicago/Turabian StyleLarsen, Sylvester, Johanne Davidsen, Katja Dahlgaard, Ole B. Pedersen, and Jesper T. Troelsen. 2019. "HNF4α and CDX2 Regulate Intestinal YAP1 Promoter Activity" International Journal of Molecular Sciences 20, no. 12: 2981. https://doi.org/10.3390/ijms20122981