ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense
Abstract
:1. Introduction
2. AOS and HPL Are Examples of Non-Canonical Cytochrome P450 Enzymes with Unique Activities in Chloroplasts
3. Structural Modelling of the LOX2–AOS–AOC2 Plastid Envelope Complex
4. Expression of LOX2, AOS, and AOC over Plant Development and Role of AOC Homo- and Hetero-Trimerization for Activity Regulation
5. Conclusions and Perspectives
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
13-HPOT | (9Z11E15Z13S)-13-hydroperoxy-9,11,15-octadecatrienoic acid |
α-LeA | α-linolenic acid |
AOC | Allene oxide cyclase |
AOS | Allene oxide synthase |
CYP | Cytochrome P450 enzymes |
EOT | 12,13(S)-epoxy-9(Z),11,15(Z)-octadecatrienoic acid |
HPL | Hydroperoxide lyase |
JA | Jasmonic acid |
LOX | Lipoxygenase |
ODA | 12-oxo-cis-9-dodecenoic acid |
OPDA | cis-(+)-12-oxophytodienoic acid |
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Amino Acid Interactions Observed in the Ternary Complex | Amino Acid Interactions Observed in an Arbitrary AOS–AOC2 Complex |
---|---|
LOX2-Ser92–AOS-Ser272 | AOS-Pro64–AOC2-Ser45 |
LOX2-Gly94–AOS-Ser272 LOX2-Asp96–AOC2-Asn42 | AOS-Ile65–AOC2-Phe44 |
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Rustgi, S.; Springer, A.; Kang, C.; von Wettstein, D.; Reinbothe, C.; Reinbothe, S.; Pollmann, S. ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense. Int. J. Mol. Sci. 2019, 20, 3064. https://doi.org/10.3390/ijms20123064
Rustgi S, Springer A, Kang C, von Wettstein D, Reinbothe C, Reinbothe S, Pollmann S. ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense. International Journal of Molecular Sciences. 2019; 20(12):3064. https://doi.org/10.3390/ijms20123064
Chicago/Turabian StyleRustgi, Sachin, Armin Springer, ChulHee Kang, Diter von Wettstein, Christiane Reinbothe, Steffen Reinbothe, and Stephan Pollmann. 2019. "ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense" International Journal of Molecular Sciences 20, no. 12: 3064. https://doi.org/10.3390/ijms20123064