Repression of the Glucocorticoid Receptor Increases Hypoxic-Ischemic Brain Injury in the Male Neonatal Rat
Abstract
:1. Introduction
2. Results
2.1. Knockdown of Glucocorticoid Receptor in the Neonatal Brain
2.2. Effect of GR Repression on HI-Induced Infarction Size in the Neonatal Brain
2.3. Effect of GR Knockdown on HI-Induced Changes in Short- and Long-Term Neurobehavioral Function in Male Pups
2.4. Effect of GR Knockdown on HI-Induced Changes in Inflammatory Cytokine Production in the Neonatal Brain
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Intracerebroventricular (ICV) Injection
4.3. Neonatal Hypoxic–Ischemic Encephalopathy (HIE) Rat Model
4.4. Measurement of Infarction Size
4.5. Neurobehavioral Tests
4.6. Real time RT-qPCR
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Knox-Concepcion, K.R.; Figueroa, J.D.; Hartman, R.E.; Li, Y.; Zhang, L. Repression of the Glucocorticoid Receptor Increases Hypoxic-Ischemic Brain Injury in the Male Neonatal Rat. Int. J. Mol. Sci. 2019, 20, 3493. https://doi.org/10.3390/ijms20143493
Knox-Concepcion KR, Figueroa JD, Hartman RE, Li Y, Zhang L. Repression of the Glucocorticoid Receptor Increases Hypoxic-Ischemic Brain Injury in the Male Neonatal Rat. International Journal of Molecular Sciences. 2019; 20(14):3493. https://doi.org/10.3390/ijms20143493
Chicago/Turabian StyleKnox-Concepcion, Katherine R., Johnny D. Figueroa, Richard E. Hartman, Yong Li, and Lubo Zhang. 2019. "Repression of the Glucocorticoid Receptor Increases Hypoxic-Ischemic Brain Injury in the Male Neonatal Rat" International Journal of Molecular Sciences 20, no. 14: 3493. https://doi.org/10.3390/ijms20143493