Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage
Abstract
:1. Introduction
2. Results and Discussion
2.1. Exogenous GA3 Application Delays Leaf Senescence of Chinese Flowering Cabbage
2.2. BrTCP21 belongs to a Member of Class I TCP
2.3. Molecular Characterization of BrTCP21
2.4. BrTCP21 Directly Binds to the Promoter of BrGA20ox3
2.5. BrTCP21 Activates the Transcription of BrGA20ox3
3. Materials and Methods
3.1. Plant Materials, GA Treatment and Growth Conditions
3.2. Physiological Measurements of Leaf Senescence
3.3. RNA Extraction, Gene Cloning and Bioinformatic Analysis
3.4. Gene Expression via qRT-PCR
3.5. Subcellular Localization Assay
3.6. Recombinant Protein Induction, Purification and EMSA Assay
3.7. Transient Transcription Dual-Luciferase (Dual-LUC) Assays
3.8. Statistical Analysis
3.9. Primers
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Xiao, X.-m.; Xu, Y.-m.; Zeng, Z.-x.; Tan, X.-l.; Liu, Z.-l.; Chen, J.-w.; Su, X.-g.; Chen, J.-y. Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage. Int. J. Mol. Sci. 2019, 20, 3860. https://doi.org/10.3390/ijms20163860
Xiao X-m, Xu Y-m, Zeng Z-x, Tan X-l, Liu Z-l, Chen J-w, Su X-g, Chen J-y. Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage. International Journal of Molecular Sciences. 2019; 20(16):3860. https://doi.org/10.3390/ijms20163860
Chicago/Turabian StyleXiao, Xian-mei, Yan-mei Xu, Ze-xiang Zeng, Xiao-li Tan, Zong-li Liu, Jian-wen Chen, Xin-guo Su, and Jian-ye Chen. 2019. "Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage" International Journal of Molecular Sciences 20, no. 16: 3860. https://doi.org/10.3390/ijms20163860