Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus

Zhang, Hai; Cheng, Guangyuan; Yang, Zongtao; Wang, Tong; Xu, Jingsheng

doi:10.3390/ijms20163867

Open AccessArticle

Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus

by

Hai Zhang

¹,

Guangyuan Cheng

¹,

Zongtao Yang

¹,

Tong Wang

¹ and

Jingsheng Xu

^1,2,*

¹

National Engineering Research Center for Sugarcane, Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

²

State Key Laboratory for Protection and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(16), 3867; https://doi.org/10.3390/ijms20163867

Submission received: 22 July 2019 / Revised: 3 August 2019 / Accepted: 6 August 2019 / Published: 8 August 2019

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Abstract

The 6K2 protein of potyviruses plays a key role in the viral infection in plants. In the present study, the coding sequence of 6K2 was cloned from Sugarcane mosaic virus (SCMV) strain FZ1 into pBT3-STE to generate the plasmid pBT3-STE-6K2, which was used as bait to screen a cDNA library prepared from sugarcane plants infected with SCMV based on the DUALmembrane system. One hundred and fifty-seven positive colonies were screened and sequenced, and the corresponding full-length genes were cloned from sugarcane cultivar ROC22. Then, 24 genes with annotations were obtained, and the deduced proteins were classified into three groups, in which eight proteins were involved in the stress response, 12 proteins were involved in transport, and four proteins were involved in photosynthesis based on their biological functions. Of the 24 proteins, 20 proteins were verified to interact with SCMV-6K2 by yeast two-hybrid assays. The possible roles of these proteins in SCMV infection on sugarcane are analyzed and discussed. This is the first report on the interaction of SCMV-6K2 with host factors from sugarcane, and will improve knowledge on the mechanism of SCMV infection in sugarcane.

Keywords: sugarcane mosaic virus; 6K2; yeast two-hybrid; interaction

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MDPI and ACS Style

Zhang, H.; Cheng, G.; Yang, Z.; Wang, T.; Xu, J. Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus. Int. J. Mol. Sci. 2019, 20, 3867. https://doi.org/10.3390/ijms20163867

AMA Style

Zhang H, Cheng G, Yang Z, Wang T, Xu J. Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus. International Journal of Molecular Sciences. 2019; 20(16):3867. https://doi.org/10.3390/ijms20163867

Chicago/Turabian Style

Zhang, Hai, Guangyuan Cheng, Zongtao Yang, Tong Wang, and Jingsheng Xu. 2019. "Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus" International Journal of Molecular Sciences 20, no. 16: 3867. https://doi.org/10.3390/ijms20163867

APA Style

Zhang, H., Cheng, G., Yang, Z., Wang, T., & Xu, J. (2019). Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus. International Journal of Molecular Sciences, 20(16), 3867. https://doi.org/10.3390/ijms20163867

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Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus

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