Comparative Analysis of miRNA Abundance Revealed the Function of Vvi-miR828 in Fruit Coloring in Root Restriction Cultivation Grapevine (Vitis vinifera L.)
Abstract
:1. Introduction
2. Result
2.1. Measurement of Soluble Solids, Titratable Acid, pH, and Anthocyanin Content in Grape Berries
2.2. Sequencing and Annotation of Grape Fruit Small RNAs
2.3. Known and Novel miRNAs
2.4. Differentially Expressed miRNAs Between RR and CK
2.5. Annotation of Potential Targets of Differentially Expressed miRNAs
2.6. Phenotypic Characterization of Vvi-miR828-OE Lines
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Measurement of the Fruit Size
4.3. Determination of Fruit Quality Index
4.4. RNA Extraction and cDNA Synthesis
4.5. Small RNA Library Construction and Sequencing
4.6. Bioinformatic Analysis of miRNAs
4.7. Analysis of Differentially Expressed miRNAs
4.8. Prediction of Target Genes for Known and Novel mRNAs and GO Analyses
4.9. Quantitative RT-PCR (qRT-PCR) Analysis
4.10. Plant Material and Growth Conditions
4.11. Construction of Plant Expression Cassettes, Plant Transformation and Characterization of Transgenic Plants
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Chen, Q.; Deng, B.; Gao, J.; Zhao, Z.; Chen, Z.; Song, S.; Wang, L.; Zhao, L.; Xu, W.; Zhang, C.; et al. Comparative Analysis of miRNA Abundance Revealed the Function of Vvi-miR828 in Fruit Coloring in Root Restriction Cultivation Grapevine (Vitis vinifera L.). Int. J. Mol. Sci. 2019, 20, 4058. https://doi.org/10.3390/ijms20164058
Chen Q, Deng B, Gao J, Zhao Z, Chen Z, Song S, Wang L, Zhao L, Xu W, Zhang C, et al. Comparative Analysis of miRNA Abundance Revealed the Function of Vvi-miR828 in Fruit Coloring in Root Restriction Cultivation Grapevine (Vitis vinifera L.). International Journal of Molecular Sciences. 2019; 20(16):4058. https://doi.org/10.3390/ijms20164058
Chicago/Turabian StyleChen, Qiuju, Bohan Deng, Jie Gao, Zhongyang Zhao, Zili Chen, Shiren Song, Lei Wang, Liping Zhao, Wenping Xu, Caixi Zhang, and et al. 2019. "Comparative Analysis of miRNA Abundance Revealed the Function of Vvi-miR828 in Fruit Coloring in Root Restriction Cultivation Grapevine (Vitis vinifera L.)" International Journal of Molecular Sciences 20, no. 16: 4058. https://doi.org/10.3390/ijms20164058