Structure Determination by Single-Particle Cryo-Electron Microscopy: Only the Sky (and Intrinsic Disorder) is the Limit
Abstract
:1. Introduction: Brief Historic Overview of Methods of Structural Biology
2. Cryo-EM: Single-Particle Analysis (SPA)
2.1. Specimen Preparation for Single Particle Cryo-EM Analysis
2.2. Data Acquisition and Image Processing
2.3. 3D-Reconstruction and Structure Validation
3. Single-Particle Cryo-EM Analysis of Membrane Proteins
3.1. Ryanodine Receptor (RyR) Channels
3.2. ATPases/V-ATPases
3.3. Transient Receptor Potential (TRP) Channels
3.4. Potassium Channels
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Nwanochie, E.; Uversky, V.N. Structure Determination by Single-Particle Cryo-Electron Microscopy: Only the Sky (and Intrinsic Disorder) is the Limit. Int. J. Mol. Sci. 2019, 20, 4186. https://doi.org/10.3390/ijms20174186
Nwanochie E, Uversky VN. Structure Determination by Single-Particle Cryo-Electron Microscopy: Only the Sky (and Intrinsic Disorder) is the Limit. International Journal of Molecular Sciences. 2019; 20(17):4186. https://doi.org/10.3390/ijms20174186
Chicago/Turabian StyleNwanochie, Emeka, and Vladimir N. Uversky. 2019. "Structure Determination by Single-Particle Cryo-Electron Microscopy: Only the Sky (and Intrinsic Disorder) is the Limit" International Journal of Molecular Sciences 20, no. 17: 4186. https://doi.org/10.3390/ijms20174186