Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria
Abstract
:1. Introduction
2. Results and Discussion
2.1. Complex Formation of KaiC with KaiB Mutants
2.2. Complex Formation of KaiC with SasA
2.3. Effects of KaiB Mutantation on Circadian Oscillations
3. Materials and Methods
3.1. Protein Expression and Purification
3.2. Size Exclusion Chormatography
3.3. Circular Dichroism Spectra
3.4. Native MS Analysis
3.5. Measurement of Time-dependent Phosphorylated KaiC Levels
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ATP | adenosine 5′-triphosphate |
p-KaiC | phosphorylated KaiC |
MS | mass spectrometry |
Te | Thermosynechococcus elongatus (strain BP-I) |
Sy | Synechococcus elongatus (strain PCC 7942) |
SDS-PAGE | sodium dodecyl sulfate polyacrylamide gel electrophoresis |
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Murakami, R.; Yunoki, Y.; Ishii, K.; Terauchi, K.; Uchiyama, S.; Yagi, H.; Kato, K. Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria. Int. J. Mol. Sci. 2019, 20, 4550. https://doi.org/10.3390/ijms20184550
Murakami R, Yunoki Y, Ishii K, Terauchi K, Uchiyama S, Yagi H, Kato K. Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria. International Journal of Molecular Sciences. 2019; 20(18):4550. https://doi.org/10.3390/ijms20184550
Chicago/Turabian StyleMurakami, Reiko, Yasuhiro Yunoki, Kentaro Ishii, Kazuki Terauchi, Susumu Uchiyama, Hirokazu Yagi, and Koichi Kato. 2019. "Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria" International Journal of Molecular Sciences 20, no. 18: 4550. https://doi.org/10.3390/ijms20184550