Zerumbone Suppresses Enterotoxigenic Bacteroides fragilis Infection-Induced Colonic Inflammation through Inhibition of NF-κΒ
Abstract
:1. Introduction
2. Results
2.1. Zerumbone Reduced Indirect Parameters of ETBF-Induced Colonic Inflammation in Mice
2.2. Zerumbone Decreased ETBF-Induced Histologic Damage in Mouse Colon
2.3. Zerumbone Decreased Infiltration of Macrophage in Distal Colon of ETBF-Infected Mice
2.4. Zerumbone Reduced Expression of Pro-Inflammatory Cytokines and NF-κB Pathway Related Proteins in Colon of ETBF-Infected Mice
2.5. Zerumbone Inhibited NF-κB Signaling and IL-8 Expression, but not Cleavage of E-Cadherin, by BFT in HT29/C1 Cells
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Cell Culture
4.3. ETBF Infection in Mice and Zerumbone Treatment
4.4. Western Blot Analysis
4.5. Quantitative RT-PCR (qRT-PCR)
4.6. Trypan Blue Exclusion Assay
4.7. ELISA
4.8. Nitric Oxide Assay
4.9. Histologic Assessment of Colonic Inflammation
4.10. Immunohistochemistry
4.11. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Hwang, S.; Jo, M.; Hong, J.E.; Park, C.O.; Lee, C.G.; Yun, M.; Rhee, K.-J. Zerumbone Suppresses Enterotoxigenic Bacteroides fragilis Infection-Induced Colonic Inflammation through Inhibition of NF-κΒ. Int. J. Mol. Sci. 2019, 20, 4560. https://doi.org/10.3390/ijms20184560
Hwang S, Jo M, Hong JE, Park CO, Lee CG, Yun M, Rhee K-J. Zerumbone Suppresses Enterotoxigenic Bacteroides fragilis Infection-Induced Colonic Inflammation through Inhibition of NF-κΒ. International Journal of Molecular Sciences. 2019; 20(18):4560. https://doi.org/10.3390/ijms20184560
Chicago/Turabian StyleHwang, Soonjae, Minjeong Jo, Ju Eun Hong, Chan Oh Park, Chang Gun Lee, Miyong Yun, and Ki-Jong Rhee. 2019. "Zerumbone Suppresses Enterotoxigenic Bacteroides fragilis Infection-Induced Colonic Inflammation through Inhibition of NF-κΒ" International Journal of Molecular Sciences 20, no. 18: 4560. https://doi.org/10.3390/ijms20184560