Cytoprotective Preconditioning of Osteoblast-Like Cells with N-Acetyl-L-Cysteine for Bone Regeneration in Cell Therapy
Abstract
:1. Introduction
2. Results
2.1. Determination of the Concentration and Incubation Time of NAC Preconditioning for Osteoblast-Like Cells
2.2. Effects of Preconditioning Osteoblast-Like Cells with NAC on Cell Viability under Oxidative Stress
2.3. Effects of Preconditioning Osteoblast-Like Cells with NAC on Cellular Redox Balance under Oxidative Stress
2.4. Effects of Preconditioning Osteoblast-Like Cells with NAC on Proliferation under Oxidative Stress
2.5. Effects of Preconditioning Osteoblast-Like Cells with NAC on Osteogenic Differentiation under Oxidative Stress
2.6. Effects of Preconditioning Osteoblast-Like Cells with NAC on Osteogenic Differentiation
2.7. Effects of Preconditioning Osteoblast-Like Cells with NAC on Bone Regeneration in Autologous Local Cell Transplantation
2.8. Histological Features of Newly Formed Bone Tissue after Autologous Local Transplantation of Osteoblast-Like Cells with NAC
3. Discussion
4. Material and Methods
4.1. Reagent Preparation and Application
4.2. Osteoblastic Cell Culture
4.3. Cell Attachment, Cytomorphometry, and Proliferation Assay
4.4. Flow Cytometry for Apoptosis Detection
4.5. GSH Detection Assay
4.6. Intracellular ROS Level
4.7. Gene Expression Analysis
4.8. Alkaline Phosphatase Activity
4.9. Matrix Mineralization Assay
4.10. Autologous Local Transplantation in A Massive Rat Femur Bone Defect
4.11. Micro-Computed Tomography Analysis
4.12. Histological Analysis
4.13. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yamada, M.; Watanabe, J.; Ueno, T.; Ogawa, T.; Egusa, H. Cytoprotective Preconditioning of Osteoblast-Like Cells with N-Acetyl-L-Cysteine for Bone Regeneration in Cell Therapy. Int. J. Mol. Sci. 2019, 20, 5199. https://doi.org/10.3390/ijms20205199
Yamada M, Watanabe J, Ueno T, Ogawa T, Egusa H. Cytoprotective Preconditioning of Osteoblast-Like Cells with N-Acetyl-L-Cysteine for Bone Regeneration in Cell Therapy. International Journal of Molecular Sciences. 2019; 20(20):5199. https://doi.org/10.3390/ijms20205199
Chicago/Turabian StyleYamada, Masahiro, Jun Watanabe, Takeshi Ueno, Takahiro Ogawa, and Hiroshi Egusa. 2019. "Cytoprotective Preconditioning of Osteoblast-Like Cells with N-Acetyl-L-Cysteine for Bone Regeneration in Cell Therapy" International Journal of Molecular Sciences 20, no. 20: 5199. https://doi.org/10.3390/ijms20205199