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Article

Analysis of the Differential Exosomal miRNAs of DC2.4 Dendritic Cells Induced by Toxoplasma gondii Infection

Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(21), 5506; https://doi.org/10.3390/ijms20215506
Submission received: 24 October 2019 / Accepted: 1 November 2019 / Published: 5 November 2019
(This article belongs to the Section Molecular Biology)

Abstract

Toxoplasma gondii is an intracellular parasite that infects humans and other warm-blooded animals. Exosomes are endocytic-derived vesicles released by cells, representing an important mode of intercellular communication. In exosomes, specific molecules of proteins, lipids, and mRNAs or miRNAs have been detected, some of which are capable of transferring biologically active molecules to recipient cells. Dendritic cells (DCs) are the only antigen-presenting cells (APCs) that activate the initial immune response. In this study, high-throughput sequencing was used to analyze the exosomal miRNA profile of DC2.4 cells infected with Toxoplasma gondii for 28 h, compared with those of uninfected DC2.4 cells. Differential exosomal miRNAs (DEmiRs) from these two cell groups were analyzed. Through high-throughput sequencing, 3434 DEmiRs were obtained, and 12 stably enriched DEmiRNAs were verified by Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) and selected for further analysis. The target genes of these 12 miRNAs were predicted with online analysis software and subjected to bioinformatics analyses including protein–protein interaction (PPI) network analysis, key driver analysis (KDA), gene ontology (GO) enrichment, and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. These DEmiRs were found to be associated with a variety of biological processes and signaling pathways involved in host ubiquitin system, innate immunity, biosynthesis, and transferase activity and could be potential biomarkers for T. gondii infection.
Keywords: Toxoplasma gondii; dendritic cells; exosome; miRNA Toxoplasma gondii; dendritic cells; exosome; miRNA
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MDPI and ACS Style

Li, D.-L.; Zou, W.-H.; Deng, S.-Q.; Peng, H.-J. Analysis of the Differential Exosomal miRNAs of DC2.4 Dendritic Cells Induced by Toxoplasma gondii Infection. Int. J. Mol. Sci. 2019, 20, 5506. https://doi.org/10.3390/ijms20215506

AMA Style

Li D-L, Zou W-H, Deng S-Q, Peng H-J. Analysis of the Differential Exosomal miRNAs of DC2.4 Dendritic Cells Induced by Toxoplasma gondii Infection. International Journal of Molecular Sciences. 2019; 20(21):5506. https://doi.org/10.3390/ijms20215506

Chicago/Turabian Style

Li, Dong-Liang, Wei-Hao Zou, Sheng-Qun Deng, and Hong-Juan Peng. 2019. "Analysis of the Differential Exosomal miRNAs of DC2.4 Dendritic Cells Induced by Toxoplasma gondii Infection" International Journal of Molecular Sciences 20, no. 21: 5506. https://doi.org/10.3390/ijms20215506

APA Style

Li, D.-L., Zou, W.-H., Deng, S.-Q., & Peng, H.-J. (2019). Analysis of the Differential Exosomal miRNAs of DC2.4 Dendritic Cells Induced by Toxoplasma gondii Infection. International Journal of Molecular Sciences, 20(21), 5506. https://doi.org/10.3390/ijms20215506

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