Angiotensin III Induces JAK2/STAT3 Leading to IL-6 Production in Rat Vascular Smooth Muscle Cells
Abstract
:1. Introduction
2. Results
2.1. STAT3 Activation by Ang II and Ang III in Wistar VSMCs
2.2. STAT3 Activation by Ang III in VSMCs from SHRs
2.3. Effects of AG490 and Ang Receptor Blockers on Ang III-induced STAT3 Phosphorylation
2.4. Effects of Ang II and Ang III on Wistar VSMC IL-6 Secretion
2.5. Effects of Ang II and Ang III on SHR VSMC IL-6 Secretion
2.6. Effects of AG490, Losartan and PD123319 on Ang III-induced IL-6 Secretion
2.7. Effects of Ang II and Ang III on IL-6 mRNA Expression in Wistar VSMCs
2.8. Effects of Ang II and Ang III on IL-6 mRNA Expression in SHR VSMCs
2.9. Effects of Ang III in Wistar versus SHR VSMCs
2.10. Effects of AG490, Losartan and PD123319 on Ang III-induced IL-6 mRNA Expression
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Isolation and Culture of Primary VSMCs
4.3. Cell Lysate Preparation
4.4. Western Blotting
4.5. Total RNA Extraction and Measurement of IL-6 mRNA Levels
4.6. Enzyme-Linked Immunosorbent Assay
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
MDPI | Multidisciplinary Digital Publishing Institute |
Ang | Angiotensin |
AT1R | angiotensin type I receptor |
AT2R | angiotensin type 2 receptor |
ANOVA | analysis of variance |
BCA | bicinchoninic acid |
DMEM/F12 | Dulbecco’s modified Eagles Medium/F12 |
DMSO | dimethyl sulfoxide |
ECL | enhanced chemiluminescence |
EDTA | ethylenediamine tetraacetic acid |
ELISA | enzyme linked immunosorbent assay |
FBS | fetal bovine serum |
INF | interferon |
IL- | interleukin |
JAK2/STAT3 | Janus kinase-2/ signal transducer and activators of transcription-3 |
MAPK | mitogen activated protein kinase |
PBS | phosphate buffer saline |
qPCR | quantitative PCR |
RAAS | renin angiotensin aldosterone system |
SDS | sodium dodecyl sulfate |
SHR | spontaneously hypertensive rat |
VSMC | vascular smooth muscle cell |
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Alanazi, A.Z.; Clark, M.A. Angiotensin III Induces JAK2/STAT3 Leading to IL-6 Production in Rat Vascular Smooth Muscle Cells. Int. J. Mol. Sci. 2019, 20, 5551. https://doi.org/10.3390/ijms20225551
Alanazi AZ, Clark MA. Angiotensin III Induces JAK2/STAT3 Leading to IL-6 Production in Rat Vascular Smooth Muscle Cells. International Journal of Molecular Sciences. 2019; 20(22):5551. https://doi.org/10.3390/ijms20225551
Chicago/Turabian StyleAlanazi, Ahmed Z., and Michelle A. Clark. 2019. "Angiotensin III Induces JAK2/STAT3 Leading to IL-6 Production in Rat Vascular Smooth Muscle Cells" International Journal of Molecular Sciences 20, no. 22: 5551. https://doi.org/10.3390/ijms20225551