C2H2-Type Zinc Finger Proteins (DkZF1/2) Synergistically Control Persimmon Fruit Deastringency
Abstract
:1. Introduction
2. Results
2.1. Phylogeny and Sequence Analyses of DkZFs
2.2. Expression of DkZFs in Response to AHCA Treatment
2.3. Transcriptional Effects of DkZFs on Promoters of Deastringency-Related Genes
2.4. Synergistic Regulations of DkZF2 and DkZF1 on DkPDC2 Promoter
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Gene Isolation and Sequence Analysis
4.3. RNA Extraction and cDNA Synthesis
4.4. Oligonucleotide Primers and Real-Time PCR
4.5. Dual-Luciferase Assay
4.6. Bimolecular Fluorescence Complementation (BiFC) Assays
4.7. Yeast Two-Hybrid Assays
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
CTAB | Cetyltrimethyl Ammonium Bromide |
MEGA | Molecular Evolutionary Genetics Analysis |
RNA-seq | Ribose nucleic acid sequencing |
Y2H | Yeast two hybrid |
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Jamil, W.; Wu, W.; Gong, H.; Huang, J.-W.; Ahmad, M.; Zhu, Q.-G.; Jin, R.; Liu, X.-F.; Yin, X.-R. C2H2-Type Zinc Finger Proteins (DkZF1/2) Synergistically Control Persimmon Fruit Deastringency. Int. J. Mol. Sci. 2019, 20, 5611. https://doi.org/10.3390/ijms20225611
Jamil W, Wu W, Gong H, Huang J-W, Ahmad M, Zhu Q-G, Jin R, Liu X-F, Yin X-R. C2H2-Type Zinc Finger Proteins (DkZF1/2) Synergistically Control Persimmon Fruit Deastringency. International Journal of Molecular Sciences. 2019; 20(22):5611. https://doi.org/10.3390/ijms20225611
Chicago/Turabian StyleJamil, Wajeeha, Wei Wu, Hui Gong, Jing-Wen Huang, Mudassar Ahmad, Qing-Gang Zhu, Rong Jin, Xiao-Fen Liu, and Xue-Ren Yin. 2019. "C2H2-Type Zinc Finger Proteins (DkZF1/2) Synergistically Control Persimmon Fruit Deastringency" International Journal of Molecular Sciences 20, no. 22: 5611. https://doi.org/10.3390/ijms20225611