Epinephrine Infiltration of Adipose Tissue Impacts MCF7 Breast Cancer Cells and Total Lipid Content
Abstract
:1. Introduction
2. Results
2.1. MCF7 Cell Proliferation was Enhanced by Epinephrine-Infiltrated Adipose Tissue Conditioned Medium
2.2. MCF7 Cell Quiescence was Increased by Sphereoid Culture and Reduced by Epinephrine-Infiltrated Adipose Tissue Conditioned Medium
2.3. Epinephrine Infiltration Changed Lipid Content and Proliferative Effect of Adipose Tissue Conditioned Medium
2.4. Injection of Epinephrine-Infiltrated Adipose Tissue or Corresponding Conditioned Medium into MCF7 Tumor in Mice
3. Discussion
4. Materials and Methods
4.1. Adipose Tissue (AT)
4.2. Culture Conditions
4.3. Cell Viability
4.4. Cell Cycle Analysis
4.5. Reverse Transcription and Quantitative PCR
4.6. Breast Carcinoma Model
4.7. Histology Analysis
4.8. Lipid Analysis
4.9. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Official Symbol | Official Full Name | Reverse Primer |
---|---|---|
HPTR1 | Hypoxanthine PhosphoRibosyl Transferase 1 | CGAGCAAGACGTTCAGTCCT |
CD44 | Cluster of Differentiation 44 | CGGCAGGTTATATTCAAATCG |
TWIST1 | Twist-related protein 1 | TGCAGAGGTGTGAGGATGGTGC |
TWIST2 | Twist-related protein 2 | AGAAGGTCTGGCAATGGCAGCA |
SNAI1 | Snail family transcriptional repressor 1 | CAGCAGGTGGGCCTGGTCGTA |
CDH1 | Cadherin 1 | CCAGCGGCCCCTTCACAGTC |
MYC | Myelocytomatosis viral oncogene homolog | GATCCAGACTCTGACCTTTTGC |
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Avril, P.; Vidal, L.; Barille-Nion, S.; Le Nail, L.-R.; Redini, F.; Layrolle, P.; Pinault, M.; Chevalier, S.; Perrot, P.; Trichet, V. Epinephrine Infiltration of Adipose Tissue Impacts MCF7 Breast Cancer Cells and Total Lipid Content. Int. J. Mol. Sci. 2019, 20, 5626. https://doi.org/10.3390/ijms20225626
Avril P, Vidal L, Barille-Nion S, Le Nail L-R, Redini F, Layrolle P, Pinault M, Chevalier S, Perrot P, Trichet V. Epinephrine Infiltration of Adipose Tissue Impacts MCF7 Breast Cancer Cells and Total Lipid Content. International Journal of Molecular Sciences. 2019; 20(22):5626. https://doi.org/10.3390/ijms20225626
Chicago/Turabian StyleAvril, Pierre, Luciano Vidal, Sophie Barille-Nion, Louis-Romée Le Nail, Françoise Redini, Pierre Layrolle, Michelle Pinault, Stéphane Chevalier, Pierre Perrot, and Valérie Trichet. 2019. "Epinephrine Infiltration of Adipose Tissue Impacts MCF7 Breast Cancer Cells and Total Lipid Content" International Journal of Molecular Sciences 20, no. 22: 5626. https://doi.org/10.3390/ijms20225626