SiMYB3 in Foxtail Millet (Setaria italica) Confers Tolerance to Low-Nitrogen Stress by Regulating Root Growth in Transgenic Plants
Abstract
:1. Introduction
2. Results
2.1. RNA-Seq Analyses of Low-Nitrogen-Induced Foxtail Millet
2.2. Characteristics Analysis of 25 MYB-Like Transcription Factors in Response to Low-Nitrogen Stress in Foxtail Millet
2.3. Subcellular Localization, Expression Pattern, and Gene Functions of SiMYB3
2.4. SiMYB3 Enhanced Tolerance to Low-Nitrogen Stress by Regulating Auxin Synthesis-Related Genes, TAR2, and Low-Nitrogen Stress-Related Genes
3. Discussion
4. Materials and Methods
4.1. Swiss-Prot, GO, and KEGG Pathway Annotation
4.2. Gene Structure and cis-Acting Elements
4.3. Plant Materials and Growth Conditions
4.4. Nitrogen Treatments on Media
4.5. Field Low-Nitrogen Stress Testing of Transgenic Rice
4.6. Subcellular Localization
4.7. RNA Isolation and Quantitative Real-Time RT-PCR
4.8. Protein Purification and Electrophoretic Mobility Shift Assays (EMSA)
5. Conclusions
Supplementary Materials
Availability of data and materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
GO | Gene Ontology |
bp | Base Pair |
cDNA | Complementary DNA |
CK | Control Check |
CDS | Coding Sequence |
RT-PCR | Reverse Transcription-Polymerase Chain Reaction |
WT | Wild Type |
OE | Overexpression Line |
LR | Lateral Root |
LN | Low Nitrogen |
LP | Low Phosphorus |
LK | Low Potassium |
TF | Transcription Factor |
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Ge, L.; Dou, Y.; Li, M.; Qu, P.; He, Z.; Liu, Y.; Xu, Z.; Chen, J.; Chen, M.; Ma, Y. SiMYB3 in Foxtail Millet (Setaria italica) Confers Tolerance to Low-Nitrogen Stress by Regulating Root Growth in Transgenic Plants. Int. J. Mol. Sci. 2019, 20, 5741. https://doi.org/10.3390/ijms20225741
Ge L, Dou Y, Li M, Qu P, He Z, Liu Y, Xu Z, Chen J, Chen M, Ma Y. SiMYB3 in Foxtail Millet (Setaria italica) Confers Tolerance to Low-Nitrogen Stress by Regulating Root Growth in Transgenic Plants. International Journal of Molecular Sciences. 2019; 20(22):5741. https://doi.org/10.3390/ijms20225741
Chicago/Turabian StyleGe, Linhao, Yining Dou, Maomao Li, Pengju Qu, Zhang He, Y Liu, Zhaoshi Xu, Jun Chen, Ming Chen, and Youzhi Ma. 2019. "SiMYB3 in Foxtail Millet (Setaria italica) Confers Tolerance to Low-Nitrogen Stress by Regulating Root Growth in Transgenic Plants" International Journal of Molecular Sciences 20, no. 22: 5741. https://doi.org/10.3390/ijms20225741