Near-Infrared Photoimmunotherapy Using a Small Protein Mimetic for HER2-Overexpressing Breast Cancer
Abstract
:1. Introduction
2. Results
2.1. Human Epidermal Growth Factor Receptor 2 (HER2) Expression
2.2. Fluorescence Image of the Cells Bound HER2 Affibody-IR700Dye Conjugate
2.3. Cell Viability after Near-Infrared Photoimmunotherapy (NIR-PIT)
2.4. Cell Images Before and after NIR-PIT by HER2 Affibobdy–IR700Dye Conjugate
2.5. NIR-PIT by HER2 Affibody–IR700Dye Conjugate Causes Necrotic Cell Death
2.6. Calcein AM and Propidium Iodide (PI) Staining for NIR-PIT-Treated Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Immunocytochemistry (ICC)
4.3. Western Blot Analysis
4.4. HER2 Affibody–IR700Dye Conjugate
4.5. Confocal Microscopy Imaging of HER2 Affibody–IR700Dye Conjugate Staining
4.6. Cell Viability Assay
4.7. Cell Images Before and after Near-Infrared (NIR) Light Irradiation
4.8. Cell Apoptosis/Necrotic Assay
4.9. Calcein AM/Propidium Iodide (PI) Staining of Mixed Cell Lines
4.10. Near-Infrared Photoimmunotherapy (NIR-PIT) Illuminator
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
HER2 | Human epidermal growth factor type 2 |
EGFR | Epidermal growth factor receptor |
NIR-PIT | Near-infrared photoimmunotherapy |
mAb | Monoclonal antibody |
ICC | Immunocytochemistry |
PI | Propidium iodide |
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Yamaguchi, H.; Pantarat, N.; Suzuki, T.; Evdokiou, A. Near-Infrared Photoimmunotherapy Using a Small Protein Mimetic for HER2-Overexpressing Breast Cancer. Int. J. Mol. Sci. 2019, 20, 5835. https://doi.org/10.3390/ijms20235835
Yamaguchi H, Pantarat N, Suzuki T, Evdokiou A. Near-Infrared Photoimmunotherapy Using a Small Protein Mimetic for HER2-Overexpressing Breast Cancer. International Journal of Molecular Sciences. 2019; 20(23):5835. https://doi.org/10.3390/ijms20235835
Chicago/Turabian StyleYamaguchi, Haruka, Namfon Pantarat, Takamasa Suzuki, and Andreas Evdokiou. 2019. "Near-Infrared Photoimmunotherapy Using a Small Protein Mimetic for HER2-Overexpressing Breast Cancer" International Journal of Molecular Sciences 20, no. 23: 5835. https://doi.org/10.3390/ijms20235835