Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis
Abstract
:1. Introduction
2. Results
2.1. Pathogenicity of the Two B. sorokiniana Strains
2.2. The Metabolomics Analysis of the Two B. sorokiniana Strains
2.3. Annotation of the Assembled Unigenes in Transcriptomes of B. sorokiniana-Infected Wheat
2.4. The Differential Expression of Pathogenicity-Related Unigenes
2.5. Unigenes Encoding Cell Wall-Degrading Enzymes
2.6. Unigenes Related to Toxin Production
2.7. The Validation of Differential Unigenes in CK, PB and WB Groups
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Strains
4.2. Pathogenicity Testing of the Two B. sorokiniana Strains
4.3. Total RNA Extraction and Sample Preparation for RNA-Seq
4.4. Assembly, Comparative Analysis, and Functional Annotation of the Transcriptome
4.5. GO Classification and Pathway Enrichment Analyses
4.6. Comparative Expression Analysis
4.7. Validation of the Gene Expression
4.8. The Metabolomics Analysis of the Two B. sorokiniana Strains
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Ye, W.; Liu, T.; Zhang, W.; Li, S.; Zhu, M.; Li, H.; Kong, Y.; Xu, L. Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis. Int. J. Mol. Sci. 2019, 20, 6090. https://doi.org/10.3390/ijms20236090
Ye W, Liu T, Zhang W, Li S, Zhu M, Li H, Kong Y, Xu L. Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis. International Journal of Molecular Sciences. 2019; 20(23):6090. https://doi.org/10.3390/ijms20236090
Chicago/Turabian StyleYe, Wei, Taomei Liu, Weimin Zhang, Saini Li, Muzi Zhu, Haohua Li, Yali Kong, and Liqiong Xu. 2019. "Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis" International Journal of Molecular Sciences 20, no. 23: 6090. https://doi.org/10.3390/ijms20236090