Combined De Novo Transcriptome and Metabolome Analysis of Common Bean Response to Fusarium oxysporum f. sp. phaseoli Infection
Abstract
:1. Introduction
2. Results
2.1. RNA Sequencing and Identification of Differentially Expressed Genes
2.2. Differential Gene Expression Analysis Related to FOP Infection
2.2.1. Structural Defense
2.2.2. Signaling
2.3. Validation of DEGs by qRT-PCR
2.4. Metabolite Profile
3. Discussion
3.1. Structural Defense in Response to FOP Infection in Common Bean
3.2. Modulation of Defense Related Proteins in Common Bean
3.3. Crucial Role of Hormones in Signaling Pathways in Common Bean-FOP Pathosystem
3.4. FOP Induced Energy Metabolism and Nitrogen Mobilization
3.5. FOP Resistance in Common Bean is Mediated by Flavonoid Biosynthesis Pathway
4. Materials and Methods
4.1. Plant Growth and In Vivo Inoculations
4.2. RNA Extraction, cDNA Library Construction, and Transcriptome Sequencing
4.3. De Novo Assembly, Functional Annotation, Classification, and Metabolic Pathway Analysis
4.4. Differential Expression and Enrichment Analysis
4.5. Quantitative RT-PCR Analysis
4.6. Widely Targeted Metabolomics
4.7. Sample Preparation
4.8. Chromatographic Mass Spectrometry Acquisition Conditions
4.9. Metabolomics Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Availability of Data
References
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Chen, L.; Wu, Q.; He, W.; He, T.; Wu, Q.; Miao, Y. Combined De Novo Transcriptome and Metabolome Analysis of Common Bean Response to Fusarium oxysporum f. sp. phaseoli Infection. Int. J. Mol. Sci. 2019, 20, 6278. https://doi.org/10.3390/ijms20246278
Chen L, Wu Q, He W, He T, Wu Q, Miao Y. Combined De Novo Transcriptome and Metabolome Analysis of Common Bean Response to Fusarium oxysporum f. sp. phaseoli Infection. International Journal of Molecular Sciences. 2019; 20(24):6278. https://doi.org/10.3390/ijms20246278
Chicago/Turabian StyleChen, Limin, Quancong Wu, Weimin He, Tianjun He, Qianqian Wu, and Yeminzi Miao. 2019. "Combined De Novo Transcriptome and Metabolome Analysis of Common Bean Response to Fusarium oxysporum f. sp. phaseoli Infection" International Journal of Molecular Sciences 20, no. 24: 6278. https://doi.org/10.3390/ijms20246278