Integrin β1-Mediated Cell–Cell Adhesion Augments Metformin-Induced Anoikis
Abstract
:1. Introduction
2. Results
2.1. Metformin Induces Cancer Cell Aggregation/Detachment and Anoikis under Glucose Restriction
2.2. Metformin Induces Expression of Integrin β1 Resulting in Cell Aggregation and Promoting Anoikis
2.3. Metformin Upregulates Integrin β1 Expression via Inhibiting ΔNp63α
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Drug Treatment
4.2. Plasmids Transfection, Lentiviral Infection, and RNA Interference
4.3. Western Blot Analyses
4.4. Quantitative PCR
4.5. FACS Assay
4.6. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ECM | Cell-extracellular matrix |
CAD | Cell Aggregation and Detachment |
HNSCC | Human head and neck squamous cell carcinoma |
NSCLC | Human non-small cell lung cancer |
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An, T.; Zhang, Z.; Li, Y.; Yi, J.; Zhang, W.; Chen, D.; Ao, J.; Xiao, Z.-X.; Yi, Y. Integrin β1-Mediated Cell–Cell Adhesion Augments Metformin-Induced Anoikis. Int. J. Mol. Sci. 2019, 20, 1161. https://doi.org/10.3390/ijms20051161
An T, Zhang Z, Li Y, Yi J, Zhang W, Chen D, Ao J, Xiao Z-X, Yi Y. Integrin β1-Mediated Cell–Cell Adhesion Augments Metformin-Induced Anoikis. International Journal of Molecular Sciences. 2019; 20(5):1161. https://doi.org/10.3390/ijms20051161
Chicago/Turabian StyleAn, Tingting, Zhiming Zhang, Yuhuang Li, Jianqiao Yi, Wenhua Zhang, Deshi Chen, Juan Ao, Zhi-Xiong Xiao, and Yong Yi. 2019. "Integrin β1-Mediated Cell–Cell Adhesion Augments Metformin-Induced Anoikis" International Journal of Molecular Sciences 20, no. 5: 1161. https://doi.org/10.3390/ijms20051161