Effects of Eleutherococcus Extract Mixture on Endochondral Bone Formation in Rats
Abstract
:1. Introduction
2. Results
2.1. HPLC Analysis of EEM Extract
2.2. Effects on Endochondral Bone Formation
2.3. Effects on GP Height
2.4. Effects on IGF1 and BMP2 Expressions in GP
2.5. Effects on IGF1 and IGFBP3 mRNA Expressions in Liver
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Sample Preparation and Quantitative Analysis
4.3. Animals
4.4. Treatment
4.5. Endochondral Bone Formation
4.6. GP Height
4.7. Immunohistochemistry
4.8. Real-Time Quantitative Polymerase Chain Reaction (PCR)
4.9. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Control | rhGH 200 μg/kg (s.c.) | EEM 50 mg/kg (p.o.) | EEM 200 mg/kg (p.o.) | |
---|---|---|---|---|
overall height of GP (μm) | 350.3 ± 19.6 | 365.4 ± 17.3 *** | 368.2 ± 27.7 * | 368.0 ± 31.4 * |
resting zone | 22.0 ± 5.3 | 21.4 ± 5.2 | 22.3 ± 5.8 | 21.1 ± 4.9 |
proliferative zone | 119.9 ± 11.5 | 127.3 ± 15.7 ** | 132.0± 12.2 ** | 125.8 ± 12.8 |
hypertrophic zone | 198.5 ± 12.7 | 203.3 ± 17.7 | 207.5 ± 22.1 | 208.7 ± 19.1 * |
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Lee, D.; Lee, S.H.; Cho, N.; Kim, Y.-S.; Song, J.; Kim, H. Effects of Eleutherococcus Extract Mixture on Endochondral Bone Formation in Rats. Int. J. Mol. Sci. 2019, 20, 1253. https://doi.org/10.3390/ijms20051253
Lee D, Lee SH, Cho N, Kim Y-S, Song J, Kim H. Effects of Eleutherococcus Extract Mixture on Endochondral Bone Formation in Rats. International Journal of Molecular Sciences. 2019; 20(5):1253. https://doi.org/10.3390/ijms20051253
Chicago/Turabian StyleLee, Donghun, Sung Hyun Lee, Namhoon Cho, Young-Sik Kim, Jungbin Song, and Hocheol Kim. 2019. "Effects of Eleutherococcus Extract Mixture on Endochondral Bone Formation in Rats" International Journal of Molecular Sciences 20, no. 5: 1253. https://doi.org/10.3390/ijms20051253