Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish
Abstract
:1. Introduction
2. Results and Discussion
2.1. Experimental Setup
2.2. Gene Biomarker Validation
2.2.1. Estrogenic and Antiestrogenic Potencies
2.2.2. Androgenic and Antiandrogenic Potencies
2.2.3. Thyroid
2.3. Screening of Compounds
2.4. Cross-Talk Effects
2.5. Zebrafish Embryos vs. Juveniles and Adults
3. Materials and Methods
3.1. Chemicals
3.2. Zebrafish Maintenance and Breeding
3.3. Toxicity Tests
3.4. EDCs Exposure Tests
3.5. Gene Expression Analysis by qPCR
3.6. Statistical Analysis
3.7. Ethical Study Approval
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
EDC | endocrine disrupting compound |
HPT | hypothalamus-pituitary-thyroid axis |
TH | thyroid hormone |
LC50 | half maximal lethal concentration |
EC50 | half maximal effective concentration |
LOEC | lowest observed effect concentration |
NOEC | no observed effect concentration |
ER | estrogen receptor |
AR | androgen receptor |
FI | fold induction |
BPA | bisphenol A |
pEDC | potential endocrine disrupting compound |
DES | Diethylstilbestrol |
END | Endosulfan |
E2 | 17β-estradiol |
FUL | Fulvestrant |
HEX | Hexaconazole |
MMI | Methimazole |
17-αMT | 17α-methyltestosterone |
NAN | Nandrolone |
NIL | Nilutamide |
TES | Testosterone |
T3 | 3,3′,5-triiodo-L-thyronine |
VIN | Vinclozolin |
DMSO | dimethyl sulfoxide |
E3 | control media |
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Parental Pathway | Compound | Estrogen Axis | Androgen Axis | Thyroid Axis |
---|---|---|---|---|
Estrogens | E2 | cyp19a1b (0.09) vtg 1 (0.19) | ||
BPA | cyp19a1b (4.99) | |||
END | vtg1 (0.33) | tpo (0.06) | ||
DES | cyp19a1b (0.01) vtg1 (0.05) | |||
Androgens | TES | cyp19a1b (1.11) vtg1 (1.46) | sult2st3 (0.14) cyp2k22 (0.74) | tpo (0.02) |
NAN | cyp19a1b (0.2) vtg1 (1.72) | sult2st3 (0.13) cyp2k22 (0.11) | dio2 (0.08) | |
17α-MT | cyp19a1b (0.62) vtg1 (2) | sult2st3 (0.10) cyp2k22 (0.05) | dio2 (0.09) | |
Thyroid | T3 | not evaluated | tpo (0.01) trα (0.038) ttr (0.0003) dio2 (0.0003) | |
MMI | tpo (397) | |||
HEX | not evaluated | dio2 (2.22) |
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Jarque, S.; Ibarra, J.; Rubio-Brotons, M.; García-Fernández, J.; Terriente, J. Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish. Int. J. Mol. Sci. 2019, 20, 1739. https://doi.org/10.3390/ijms20071739
Jarque S, Ibarra J, Rubio-Brotons M, García-Fernández J, Terriente J. Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish. International Journal of Molecular Sciences. 2019; 20(7):1739. https://doi.org/10.3390/ijms20071739
Chicago/Turabian StyleJarque, Sergio, Jone Ibarra, Maria Rubio-Brotons, Jessica García-Fernández, and Javier Terriente. 2019. "Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish" International Journal of Molecular Sciences 20, no. 7: 1739. https://doi.org/10.3390/ijms20071739