Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Character and Physiological Parameters in Maiyuanjinqiu and C. fargesii
2.2. Analyses of Global Phosphorylated Proteins and Sites in Maiyuanjinqiu and C. fargesii
2.3. Secondary Structure and Motif Analysis of Phosphoproteins
2.4. Identification of Differential Phosphoproteins (DPs)
2.5. Gene Ontology (GO) Functional Classification, Subcellular Localization, and GO Enrichment Analysis
2.6. Domain Enrichment Analysis
2.7. KEGG Pathway of Differential Phosphoproteins in Different Sectors of Maiyuanjinqiu
2.8. Protein–Protein Interaction (PPI) Network of Phosphoproteins
2.9. The Key Enzyme Activities
3. Discussion
3.1. Global Analysis of Phosphorylome Profiling Associated with Leaf Color
3.2. DPs Involved in Pigment Biosynthesis and Photosynthesis
3.3. DPs Involved in Energy Metabolism
3.4. DPs Involved in Stress Response and Defense
3.5. DPs Involved in Protein Homeostasis
3.6. DPs Involved in Transcriptional Regulation and Transport
4. Materials and Methods
4.1. Plant Material
4.2. Determination of Photosynthetic Fluorescence Parameters and Pigment Contents
4.3. Protein Extraction, Trypsin Digestion, and TMT Labeling
4.4. HPLC Fractionation and Affinity Enrichment
4.5. LC-MS/MS Analysis
4.6. Database Search and Data Analysis
4.7. Bioinformatics Methods
4.8. Key Enzyme Activities
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
Fv/Fm | Maximum quantum yield of PSII |
ETR | Electron transport rate |
NPQ | Non photochemical quenching |
PS | Photosystem |
LC-MS/MS | Liquid chromatography–mass spectrometry/mass spectrometry |
GO | Gene ontology |
DPs | Differential phosphoproteins |
LHCB | Chlorophyll a-b binding protein |
ATPF 1G | ATP synthase subunit gamma |
PsbO | Oxygen-evolving enhancer protein 1 |
PsbP | Oxygen-evolving enhancer protein 2 |
PsbQ | Oxygen-evolving enhancer protein 3 |
PsbR | Photosystem II reaction center protein H |
PsbH | Photosystem II reaction center protein H |
LHCB | Chlorophyll a-b binding protein |
PsaE | Putative Photosystem I reaction center subunit IV |
PsaF | Putative photosystem I reaction center subunit III |
PC | Plastocyanin |
GluTR | Glutamyl-tRNA reductase |
NCED | 9-cis-epoxycarotenoid dioxygenase 1 |
P450 | Cytochrome P450 family protein, expressed |
3.1.3.37 | Putative fructose-1,6-bisphosphatase |
PRK | Phosphoribulokinase |
rbcL | Ribulose bisphosphate carboxylase large chain |
ALDO | Fructose-bisphosphate aldolase |
GAPA | EST C74302 (E30840) corresponds to a region of the predicted gene |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic |
PGK | Phosphoenolpyruvate carboxykinase |
PK | Pyruvate kinase |
gpml | Putative 2, 3-bisphosphoglycerate-independent phosphoglycerate mutase |
glgC | Glucose-1-phosphate adenylyltransferase |
Glu | Glutamine syntheses |
TrpA | Tryptophan synthase alpha chain |
GLDC | Putative glycine dehydrogenase |
GGAT | Alanine aminotransferase |
gcvT | Aminomethyltransferase |
AGXT | Serine-glyoxylate aminotransferase |
asd | Aspartate-semialdehyde dehydrogenase family protein, expressed |
SNF2 | SNF2 transcription factor |
MYB | MYB transcription factor |
H2B | Histone H2B |
APX | Ascorbate peroxidase |
RP | Ribosomal protein |
EIF | Eukaryotic translation initiation factor |
Clp | ATP-dependent Clp protease proteolytic subunit |
FTSH1 | ATP-dependent zinc metalloprotease FTSH 1, chloroplastic |
DNM | Putative dynamin homolog |
GIDIL | Gibberellin receptor GID1L2 |
lacZ | Glycoside hydrolase family 2 family protein |
PMA | H-ATPase |
ALAD | ALA dehydrogenase |
PBGD | Porphobilinogen deaminase |
CPOX | Coproporphyrinogen III oxidase |
PPOX | Protoporphyrinogen IX oxidase |
SOD | Superoxide dismutase |
ELISA | Enzyme-linked immunosorbent assay |
Appendix A
Protein Description (Abbreviation/Phosphorylated Amino Acid/Site) | G1 | G2 | Y1 | Y2 |
---|---|---|---|---|
Pigment biosynthesis | ||||
Glutamyl-tRNA reductase (GluTR/S/55) | 0.53 | 0.69 | 2.37 | 0.92 |
Porphobilinogen deaminase, chloroplastic (hemC/T/339) | 0.72 | 0.78 | 1.34 | 1.17 |
9-cis-epoxycarotenoid dioxygenase 1 (NCED/S/51) | 0.42 | 0.44 | 1.66 | 1.84 |
Cytochrome P450 family protein, expressed (P450/S/251) | 0.53 | 0.74 | 1.17 | 1.52 |
Cytochrome P450 family protein, expressed (P450/S/134) | 0.46 | 0.69 | 1.08 | 1.62 |
Photosynthesis | ||||
Oxygen-evolving enhancer protein 1 (PsbO/Y/252) | 0.26 | 0.34 | 3.29 | 0.95 |
Oxygen-evolving enhancer protein 1 (PsbO/S/239) | 0.35 | 0.48 | 3.12 | 0.84 |
Oxygen-evolving enhancer protein 1 (PsbO/S/174) | 0.20 | 0.27 | 3.02 | 0.88 |
Oxygen-evolving enhancer protein 1 (PsbO/S/173) | 0.40 | 0.46 | 2.73 | 0.76 |
Oxygen-evolving enhancer protein 1 (PsbO/S/141) | 0.50 | 0.67 | 2.65 | 0.80 |
Oxygen-evolving enhancer protein 1 (PsbO/Y/187) | 0.37 | 0.48 | 2.64 | 0.83 |
Oxygen-evolving enhancer protein 1 (PsbO/S/88) | 0.77 | 0.99 | 1.46 | 0.90 |
Oxygen-evolving enhancer protein 1 (PsbO/S/153) | 0.77 | 0.82 | 1.35 | 1.18 |
Oxygen-evolving enhancer protein 1 (PsbO/S/249) | 0.86 | 0.97 | 1.34 | 0.95 |
Oxygen-evolving enhancer protein 1 (PsbO/S/238) | 0.16 | 0.21 | 3.65 | 0.96 |
Oxygen-evolving enhancer protein 2 (PsbP/S/50) | 0.26 | 0.30 | 5.13 | 0.94 |
Oxygen-evolving enhancer 2-1, chloroplastic (PsbP/Y/21) | 0.89 | 0.95 | 1.71 | 1.04 |
Oxygen-evolving enhancer 2-1, chloroplastic (PsbP/T/22) | 0.89 | 0.94 | 1.64 | 1.07 |
Oxygen-evolving enhancer protein 3-1 (PsbQ/T/123) | 0.84 | 0.81 | 2.54 | 1.28 |
Oxygen-evolving enhancer protein 3-1 (PsbQ/Y/118) | 0.82 | 0.92 | 1.30 | 1.39 |
Photosystem II reaction center protein H (PsbH/T/3) | 1.05 | 1.18 | 0.85 | 0.76 |
Photosystem II reaction center protein H (PsbH/T/5) | 0.95 | 1.19 | 2.08 | 0.68 |
Photosystem II 10 kDa polypeptide (PsbR/Y/74) | 0.79 | 1.13 | 1.64 | 0.93 |
Plastocyanin (PetE/S/154) | 0.79 | 0.88 | 1.32 | 0.85 |
Putative photosystem I reaction center subunit III (PsaF/S/100) | 0.69 | 0.78 | 3.53 | 1.38 |
Putative Photosystem I reaction center subunit IV (PsaE/S/93) | 0.42 | 0.50 | 3.46 | 1.37 |
Putative Photosystem I reaction center subunit IV (PsaE/S/101) | 0.74 | 0.77 | 2.31 | 1.05 |
Putative photosystem I reaction center subunit III (PsaF/S/99) | 0.69 | 0.78 | 3.39 | 1.35 |
ATP synthase subunit gamma (ATPF1G/S/348) | 0.53 | 0.65 | 3.33 | 1.13 |
ATP synthase subunit gamma (ATPF1G/Y/351) | 0.55 | 0.66 | 3.28 | 1.11 |
ATP synthase subunit gamma (ATPF1G/S/99) | 0.55 | 0.66 | 3.28 | 1.11 |
Photosynthesis –antenna proteins | ||||
Chlorophyll a/b-binding protein (LHCB4/S/49) | 0.94 | 0.97 | 2.15 | 0.91 |
Chlorophyll a/b-binding protein (LHCB4/S/49) | 0.94 | 0.97 | 2.15 | 0.91 |
Chlorophyll a-b binding protein 2, chloroplastic (LHCB1/S/38) | 0.76 | 0.89 | 3.90 | 0.90 |
Chlorophyll a-b binding protein 1(LHCB1/T/23) | 0.76 | 0.89 | 1.73 | 1.01 |
Chlorophyll a-b binding protein 1 (LHCB1/S/43) | 0.48 | 0.59 | 1.66 | 1.97 |
Chlorophyll a-b binding protein 1 (LHCB1/Y/49) | 1.14 | 1.09 | 0.59 | 0.84 |
Chlorophyll a-b binding protein 2, chloroplastic (LHCB1/S/47) | 1.01 | 1.00 | 0.45 | 1.01 |
Chlorophyll a-b binding protein 1 (LHCB1/S/41) | 1.11 | 1.08 | 0.42 | 1.00 |
Chlorophyll a-b binding protein 2, chloroplastic (LHCB1/Y/50) | 1.05 | 0.97 | 0.27 | 1.01 |
Stress and stimulus response proteins | ||||
Catalase (katE/S/347) | 0.52 | 0.59 | 2.23 | 1.12 |
Catalase (katE/S/349) | 0.49 | 0.70 | 2.16 | 1.03 |
Catalase (katE/S/112) | 0.55 | 0.66 | 2.00 | 1.15 |
Catalase (katE/T/115) | 0.59 | 0.65 | 1.95 | 1.14 |
Catalase (katE/S/11) | 0.78 | 0.85 | 1.35 | 0.98 |
Catalase (katE/T/351) | 0.47 | 0.59 | 1.85 | 1.41 |
Probable L-ascorbate peroxidase 3 (E1.11.1.11/S/150) | 0.70 | 0.74 | 2.19 | 1.25 |
OSJNBb0048E02.12 protein (OSJNBb0048E02.12/S/8) | 0.62 | 0.78 | 1.44 | 0.89 |
Glutaredoxin domain containing protein (S/154) | 0.66 | 1.02 | 1.43 | 0.94 |
OSJNBb0048E02.12 protein (OSJNBb0048E02.12/S/50) | 0.80 | 0.77 | 1.40 | 0.81 |
OSJNBb0048E02.12 protein (OSJNBb0048E02.12/Y/3) | 0.66 | 0.78 | 1.39 | 0.96 |
OSJNBb0048E02.12 protein (OSJNBb0048E02.12/S/5) | 0.68 | 0.8 | 1.36 | 0.98 |
Putative cinnamyl-alcohol dehydrogenase family protein (E1.1.1.195/T/7) | 1.00 | 1.08 | 1.39 | 0.90 |
Glutaredoxin domain containing protein (S/99) | 0.87 | 1.00 | 1.38 | 0.79 |
Glutaredoxin domain containing protein (S/46) | 0.87 | 1.06 | 0.79 | 1.27 |
Probable cinnamyl alcohol dehydrogenase 3 (S/20) | 0.90 | 1.03 | 1.38 | 0.83 |
Glutaredoxin domain containing protein (S/103) | 0.86 | 1.00 | 1.34 | 0.84 |
Putative nuclear snf4-like (S/48) | 0.91 | 0.98 | 1.32 | 0.87 |
Homeobox-leucine zipper protein ROC1 (S/46) | 1.01 | 1.08 | 4.2 | 0.40 |
Lipoxygenase (LOX2S/S/76) | 0.46 | 0.59 | 2.71 | 1.31 |
Lipoxygenase (LOX2S/S/74) | 0.44 | 0.51 | 2.7 | 1.5 |
Putative ENTH/ANTH/VHS superfamily protein isoform 1 (S/180) | 0.78 | 0.81 | 1.78 | 0.71 |
Integral membrane single C2 domain protein (T/475) | 0.83 | 0.90 | 1.66 | 0.73 |
Integral membrane single C2 domain protein (T/473) | 0.82 | 0.93 | 1.64 | 0.73 |
Clathrin assembly protein AP180 short form-like (S/306) | 0.84 | 0.91 | 1.62 | 0.78 |
Clathrin assembly protein AP180 short form-like (T/307) | 0.93 | 0.94 | 1.42 | 0.77 |
Putative ENTH/ANTH/VHS superfamily protein isoform 1 (T/221) | 0.91 | 0.93 | 1.26 | 0.78 |
Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (HAO/T/159) | 0.97 | 1.14 | 1.22 | 0.79 |
Monodehydroascorbate reductase (E1.6.5.4/S/344) | 0.74 | 1.33 | 1.17 | 0.74 |
Monodehydroascorbate reductase (E1.6.5.4/T/346) | 0.69 | 1.30 | 1.16 | 0.86 |
Chaperonin (Chaperonin/T/125) | 0.73 | 0.81 | 1.09 | 1.25 |
Glutathione peroxidase (gpx/S/129) | 0.98 | 1.19 | 0.75 | 1.33 |
Putative C2 domain-containing protein (S/406) | 0.87 | 1.05 | 0.71 | 1.32 |
L-lactate dehydrogenase (LDH/S/26) | 1.22 | 1.24 | 0.75 | 0.81 |
Energy and metabolism | ||||
Fructose-bisphosphate aldolase (ALDO/T/88) | 0.67 | 0.67 | 3.48 | 0.97 |
Fructose-bisphosphate aldolase (ALDO/S/42) | 0.56 | 0.70 | 2.72 | 0.78 |
Fructose-bisphosphate aldolase (ALDO/S/82) | 0.67 | 0.88 | 1.68 | 0.91 |
Fructose-bisphosphate aldolase (ALDO/S/73) | 0.76 | 1.06 | 1.44 | 0.93 |
Fructose-bisphosphate aldolase (ALDO/S/83) | 0.79 | 0.91 | 1.44 | 0.82 |
Fructose-bisphosphate aldolase (ALDO/S/349) | 0.92 | 0.99 | 1.42 | 0.76 |
Fructose-bisphosphate aldolase (ALDO/T/80) | 0.83 | 0.96 | 1.36 | 1.13 |
Transketolase isoform 1 (transketolase [EC:2.2.1.1]/Y/426) | 0.66 | 0.67 | 3.35 | 0.77 |
Transketolase isoform 1(transketolase ([EC:2.2.1.1]/S/434) | 0.77 | 0.89 | 1.76 | 0.78 |
Transketolase isoform 1 ( transketolase [EC:2.2.1.1]/S/430) | 0.97 | 1.14 | 1.22 | 0.83 |
EST C74302(E30840) corresponds to a region of the predicted gene (GAPA/S/26) | 0.40 | 0.57 | 2.35 | 0.79 |
EST C74302(E30840) corresponds to a region of the predicted gene (GAPA/S/27) | 0.48 | 0.59 | 2.27 | 0.82 |
EST C74302(E30840) corresponds to a region of the predicted gene (GAPA/S/25) | 0.75 | 0.82 | 1.52 | 0.81 |
EST C74302(E30840) corresponds to a region of the predicted gene (GAPA/S/278) | 0.81 | 0.90 | 1.37 | 1.14 |
EST C74302(E30840) corresponds to a region of the predicted gene (GAPA/T/295) | 0.90 | 1.29 | 1.12 | 0.99 |
putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI/S/81) | 0.84 | 0.88 | 1.53 | 0.77 |
Phosphoenolpyruvate carboxykinase (ATP), conserved site-containing protein (E4.1.1.49/T/71) | 0.76 | 1.06 | 1.44 | 0.66 |
Phosphoglucomutase/phosphomannomutase, C-terminal domain, putative (pgn/T/5) | 0.81 | 0.86 | 1.27 | 0.75 |
Carbonic anhydrase (cynT/S/111) | 0.51 | 0.66 | 2.14 | 0.75 |
Carbonic anhydrase (cynT/S/109) | 0.80 | 0.86 | 1.19 | 0.78 |
Carbonic anhydrase (cynT/S/83) | 0.84 | 1.01 | 1.52 | 1.26 |
Phosphoribulokinase (PRK/T/78) | 0.60 | 0.60 | 2.03 | 0.85 |
Phosphoribulokinase (PRK/S/219) | 0.64 | 0.80 | 1.45 | 0.94 |
Phosphoribulokinase (PRK/S/79) | 0.88 | 1.03 | 1.45 | 0.85 |
Putative fructose-1,6-bisphosphatase (E3.1.3.37/S/320) | 0.72 | 0.82 | 1.80 | 0.83 |
Serine-glyoxylate aminotransferase (AGXT/Y/35) | 0.85 | 0.87 | 1.76 | 1.02 |
Serine-glyoxylate aminotransferase (AGXT/S/37) | 0.69 | 0.73 | 1.46 | 1.00 |
Ribulose bisphosphate carboxylase small chain (rbcS/S/114) | 0.7 | 1.24 | 1.59 | 1.16 |
Ribulose bisphosphate carboxylase small chain (rbcS/Y/118) | 1.01 | 1.00 | 1.37 | 1.21 |
Ribulose bisphosphate carboxylase small chain (rbcS/Y/117) | 0.95 | 1.26 | 1.21 | 1.20 |
GLO5 Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (HAO/T/159) | 0.79 | 0.97 | 1.42 | 0.79 |
2-oxoglutarate dehydrogenase, putative (OGDH/S/344) | 0.94 | 1.01 | 1.33 | 0.91 |
Putative fructose-1,6-bisphosphatase (E3.1.3.37/S/318) | 0.88 | 0.89 | 1.31 | 1.10 |
Phosphoglycerate kinase (PGK/S/276) | 0.83 | 0.94 | 1.26 | 1.05 |
Aminomethyltransferase (gcvT/S/268) | 0.75 | 0.94 | 2.09 | 0.92 |
Aminomethyltransferase (gcvT/Y/390) | 1.02 | 1.22 | 1.10 | 1.10 |
Aminomethyltransferase (gcvT/S/339) | 0.74 | 1.02 | 0.83 | 0.82 |
Glucose-1-phosphate adenylyltransferase (glgC/S/263) | 0.39 | 0.41 | 2.13 | 1.10 |
Glucose-1-phosphate adenylyltransferase (glgC/S/424) | 0.48 | 0.47 | 2.05 | 1.05 |
UDP-glucose 6-dehydrogenase (UGDH/S/393) | 1.15 | 1.12 | 0.69 | 0.96 |
1,3-beta-glucan synthase component-like (S/1019) | 0.80 | 1.04 | 1.43 | 0.79 |
Putative beta 1,3 glucan synthase (T/27) | 0.76 | 1.05 | 1.41 | 0.91 |
Serine hydroxymethyltransferase (glyA/S/384) | 0.72 | 0.82 | 1.8 | 1.06 |
Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic (E2.5.1.54/S/67) | 0.43 | 0.47 | 1.72 | 1.10 |
Aspartate-semialdehyde dehydrogenase family protein, expressed (ASD/S/137) | 0.72 | 0.79 | 1.65 | 0.78 |
Orange pericarp1 (trpB/S/76) | 0.79 | 0.97 | 1.48 | 0.91 |
Cellulose synthase-like protein E6 (IPR029044/S/393) | 0.5 | 0.63 | 1.8 | 1.36 |
Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPDH/Y/257) | 0.75 | 0.82 | 1.52 | 0.74 |
Putative beta 1,3 glucan synthase (S/29) | 0.81 | 1.08 | 1.29 | 0.90 |
putative 6-phosphofructo-2-kinase / fructose-2, 6-bisphosphate 2-phosphatase(S/276) | 1.04 | 1.16 | 0.56 | 1.28 |
Glutamine synthetase (Gs/S/175) | 0.76 | 0.87 | 1.61 | 0.98 |
Putative glycine dehydrogenase (GLDC/S/51) | 0.77 | 0.89 | 1.76 | 0.95 |
Thiamine thiazole synthase, chloroplastic (THI4/S/27) | 0.37 | 0.37 | 2.41 | 0.62 |
Nitrogen regulatory protein P-II homolog (/T114) | 0.51 | 0.62 | 1.71 | 0.63 |
Nitrogen regulatory protein P-II homolog (/T113) | 0.57 | 0.7 | 1.61 | 0.68 |
Nitrate-induced NOI protein, expressed (RIN4/T/161) | 0.61 | 0.96 | 1.53 | 0.70 |
Nitrate-induced NOI protein, expressed (RIN4/T/206) | 0.77 | 1.13 | 1.34 | 0.64 |
Nitrate-induced NOI protein, expressed (RIN4/T/216) | 0.74 | 1.16 | 1.32 | 0.68 |
Nitrate-induced NOI protein, expressed (RIN4/T/227) | 0.79 | 1.07 | 1.22 | 0.88 |
Nitrate-induced NOI protein, expressed (RIN4/T/168) | 0.77 | 0.78 | 1.54 | 0.63 |
Nitrate-induced NOI protein, expressed (RIN4/S/170) | 0.82 | 0.74 | 1.39 | 0.68 |
Nitrate-induced NOI protein, expressed (RIN4/S/96) | 0.96 | 0.95 | 1.31 | 0.66 |
Nitrate-induced NOI protein, expressed (RIN4/T/165) | 0.79 | 0.88 | 1.4 | 0.75 |
Nitrate-induced NOI protein, expressed (RIN4/T/228) | 0.86 | 0.87 | 1.32 | 0.82 |
Nitrate-induced NOI protein, expressed (RIN4/S/154) | 0.99 | 1.03 | 1.19 | 0.77 |
Nitrate-induced NOI protein, expressed (RIN4/S/183) | 0.87 | 0.91 | 1.28 | 0.84 |
Nitrate-induced NOI protein, expressed (RIN4/S/127) | 0.93 | 0.96 | 1.23 | 0.81 |
Nitrate-induced NOI protein, expressed (RIN4/S/164) | 0.83 | 0.92 | 1.28 | 0.85 |
Nitrate-induced NOI protein, expressed (RIN4/S/225) | 0.72 | 1.31 | 1.09 | 0.92 |
Nitrate-induced NOI protein, expressed (RIN4/S/133) | 1.22 | 1.16 | 0.97 | 0.77 |
Nitrate-induced NOI protein, expressed (RIN4/S/185) | 1.59 | 1.84 | 0.4 | 0.43 |
Nitrate-induced NOI protein, expressed (RIN4/S/95) | 1.17 | 1.39 | 0.75 | 0.84 |
Protein synthesis | ||||
50S ribosomal protein L21 (RP-L21/S/52) | 0.32 | 0.38 | 2.4 | 1.18 |
60S acidic ribosomal protein P0 (RP-LP0/S/311) | 0.25 | 0.35 | 1.53 | 0.25 |
60S acidic ribosomal protein P1 (RP-LP1/S/103) | 0.23 | 0.21 | 1.51 | 0.28 |
60S ribosomal protein L6 (RP-L6e/S/204) | 0.9 | 0.92 | 1.36 | 0.86 |
60S acidic ribosomal protein P2A (RP-LP2/S/105) | 0.15 | 0.34 | 1.33 | 0.15 |
60S acidic ribosomal protein P3 (RP-LP1/S/110) | 0.35 | 0.47 | 1.31 | 0.37 |
60S ribosomal protein L24 (RP-L24e/S/28) | 0.82 | 1.09 | 1.24 | 0.86 |
40S ribosomal protein S27 (RP-S27e/S/80) | 1.15 | 1.70 | 0.65 | 0.80 |
40S ribosomal protein S6 (RP-S6e/S/231) | 1.00 | 1.17 | 0.65 | 0.96 |
40S ribosomal protein S6 (RP-S6e/S/237) | 1.02 | 1.3 | 0.51 | 0.76 |
40S ribosomal protein S6 (RP-S6e/S/229) | 1.21 | 1.32 | 0.29 | 0.56 |
Putative translational activator (S/1831) | 0.78 | 0.83 | 1.88 | 0.78 |
4G-1 Eukaryotic translation initiation factor isoform 4G-1 (EIF4G/S/527) | 0.86 | 1.11 | 1.34 | 0.74 |
4B Eukaryotic translation initiation factor 4B (EIF4B/S/128) | 0.72 | 0.84 | 1.29 | 0.86 |
3H Eukaryotic translation initiation factor 3 subunit H (EIF3H/S/222) | 0.96 | 1.27 | 0.91 | 0.79 |
4G Eukaryotic translation initiation factor 4G (EIF4G/T/1579) | 1.49 | 1.52 | 0.8 | 1.15 |
4B Eukaryotic translation initiation factor 4B (EIF4B/Y/536) | 1.15 | 1.12 | 0.73 | 1.17 |
4G Eukaryotic translation initiation factor 4G (EIF4G/S/184) | 1.74 | 1.53 | 0.73 | 1.13 |
4G Eukaryotic translation initiation factor 4G (EIF4G/S1401) | 2.24 | 1.76 | 0.59 | 1.00 |
Elongation factor 1-alpha (EEF1A/S/176) | 1.22 | 1.07 | 0.54 | 1.18 |
Protein Degradation | G1 | G2 | Y1 | Y2 |
Putative dynamin homolog (DNM/S/894) | 0.47 | 0.57 | 2.53 | 1.44 |
Putative dynamin homolog (DNM/S/897) | 0.52 | 0.60 | 2.49 | 1.38 |
Putative dynamin homolog (DNM/S/887) | 0.77 | 0.84 | 1.67 | 0.80 |
Putative dynamin homolog (DNM/Y/886) | 0.69 | 0.88 | 1.67 | 0.75 |
Putative dynamin homolog (DNM/T/736) | 0.79 | 0.88 | 1.56 | 1.11 |
Putative dynamin homolog (DNM/S/733) | 0.80 | 0.86 | 1.49 | 1.20 |
Putative dynamin homolog (DNM/S/730) | 0.80 | 0.87 | 1.48 | 1.19 |
Putative dynamin homolog (DNM/T/535) | 0.88 | 0.95 | 1.32 | 1.00 |
Dynamin-related protein 1C, putative, expressed (DNM/S/271) | 0.76 | 0.82 | 1.66 | 1.06 |
ATP-dependent Clp protease proteolytic subunit (ClpP/S/288) | 0.31 | 0.37 | 2.46 | 0.89 |
ATP-dependent Clp protease proteolytic subunit (ClpP/S/287) | 0.30 | 0.46 | 2.23 | 0.90 |
ATP-dependent zinc metalloprotease FTSH 1, chloroplastic (FTSH1/T/539) | 0.39 | 0.53 | 2.42 | 0.90 |
ATP-dependent Clp protease proteolytic subunit (ClpP/S/287) | 0.30 | 0.46 | 2.23 | 0.90 |
ATP-dependent Clp protease proteolytic subunit (ClpP/S/96) | 0.49 | 0.60 | 1.70 | 1.00 |
H-ATPase (PMA1/Y/955) | 0.83 | 0.40 | 2.02 | 1.07 |
H-ATPase (PMA1/T/956) | 0.67 | 0.80 | 2.01 | 0.79 |
Pleiotropic drug resistance protein 5 (S/26) | 0.56 | 0.77 | 1.83 | 0.98 |
Pleiotropic drug resistance protein 6 (S/14) | 0.94 | 1.05 | 1.28 | 0.73 |
Pleiotropic drug resistance protein 6 (S/12) | 0.93 | 1.08 | 1.21 | 0.79 |
Chloroplast thylakoidal processing peptidase-like protein (S/392) | 0.54 | 0.62 | 1.65 | 1.11 |
Neutral/alkaline invertase (Neutral/alkaline invertase/S/48) | 0.81 | 0.99 | 1.62 | 0.85 |
Beta-glucosidase 11 (Beta-glucosidase 11/S546) | 0.57 | 0.60 | 1.62 | 0.99 |
Endonuclease III homologue (NTH/S/346) | 0.80 | 0.82 | 1.61 | 0.83 |
Gibberellin receptor GID1L2 (GID1L2/S/309) | 0.62 | 0.71 | 1.60 | 1.25 |
Beta-glucosidase 11 (Beta-glucosidase 11/S545) | 0.60 | 0.62 | 1.58 | 0.98 |
Beta-glucosidase 11 (Beta-glucosidase 11/S555) | 0.67 | 0.72 | 1.50 | 0.93 |
Beta-glucosidase 11 (Beta-glucosidase 11/S/1007) | 0.69 | 0.77 | 1.45 | 1.13 |
Beta-glucosidase 11 (Beta-glucosidase 11/S/249) | 0.70 | 0.78 | 1.25 | 1.21 |
Beta-glucosidase 24 (Beta-glucosidase 24/S/279) | 0.66 | 0.64 | 1.47 | 0.8 |
Beta-glucosidase 24 (Beta-glucosidase 24 /Y/286) | 0.64 | 0.63 | 1.47 | 0.81 |
Beta-glucosidase 24 (Beta-glucosidase 24/S/271) | 0.66 | 0.83 | 1.35 | 0.87 |
Beta-glucosidase 24 (Beta-glucosidase 24/S/269) | 0.66 | 0.73 | 1.33 | 0.94 |
DNA topoisomerase I (DNA topoisomerase I/S/139) | 0.90 | 0.98 | 1.41 | 0.89 |
DNA topoisomerase I (DNA topoisomerase I/S/142) | 0.90 | 0.98 | 1.41 | 0.89 |
Glycoside hydrolase family 2 family protein (lacZ/S/567) | 0.68 | 0.74 | 1.35 | 1.35 |
Ubiquitin family protein (Ubiquitin family protein/T/351) | 0.93 | 0.92 | 1.33 | 0.74 |
H2B Filamentation temperature-sensitive H2B (S/198) | 0.81 | 1.02 | 1.24 | 0.95 |
H2B Filamentation temperature-sensitive H2B (S/199) | 0.81 | 1.02 | 1.24 | 0.95 |
putative endo-1,3;1,4-beta-D-glucanase (S/209) | 0.93 | 1.06 | 1.23 | 0.81 |
Beta-glucosidase-like SFR2, chloroplastic (S/132) | 0.75 | 1.11 | 0.85 | 1.30 |
Soluble inorganic pyrophosphatase (ppa/S/33) | 0.82 | 1.17 | 0.77 | 1.25 |
Soluble inorganic pyrophosphatase (ppa/S/34) | 0.80 | 1.10 | 0.77 | 1.33 |
DNA topoisomerase I (DNA topoisomerase I/S/192) | 1.11 | 1.21 | 0.71 | 0.63 |
CER5 (CER5/S/330) | 1.01 | 1.24 | 0.64 | 1.09 |
Transcriptional regulation | ||||
Histone H2B (H2B/S/135) | 0.92 | 1.02 | 1.30 | 0.77 |
Histone H2B (H2B/S/19) | 1.25 | 1.12 | 0.73 | 0.9 |
SNF2 transcription factor (MSI/S/95) | 1.28 | 1.25 | 0.72 | 1.16 |
MYB transcription factor (S/184) | 1.15 | 1.19 | 0.66 | 1.06 |
Putative MYB DNA-binding domain superfamily protein (S/171) | 0.75 | 1.02 | 1.25 | 1.01 |
Zinc finger CCCH domain-containing protein 8 (S/436) | 1.36 | 1.2 | 0.82 | 0.78 |
Zinc finger CCCH domain-containing protein 8 (S/238) | 1.27 | 1.34 | 0.59 | 1.01 |
Zinc-finger homeodomain protein 1 (S/199) | 0.70 | 0.86 | 1.30 | 1.24 |
Zinc-finger homeodomain protein 1 (T/37) | 0.78 | 1.04 | 1.22 | 1.00 |
Zinc-finger homeodomain protein 1 (T/17) | 1.01 | 0.88 | 1.40 | 0.73 |
Zinc-finger homeodomain protein 3 (Y/151) | 0.76 | 1.00 | 1.33 | 1.03 |
Zinc finger CCCH domain-containing protein 8 (S/436) | 1.36 | 1.20 | 0.82 | 0.78 |
Zinc finger CCCH domain-containing protein 8 (S/238) | 1.27 | 1.34 | 0.59 | 1.01 |
Zinc finger CCCH domain-containing protein 63 (S/408) | 0.69 | 0.97 | 1.84 | 0.85 |
Zinc finger CCCH domain-containing protein 63 (S/410) | 0.72 | 1.01 | 1.80 | 0.81 |
Zinc finger CCCH domain-containing protein 63 (S/183) | 0.76 | 1.04 | 1.76 | 0.73 |
Zinc finger CCCH domain-containing protein 63 (T/185) | 0.89 | 1.08 | 1.48 | 0.72 |
Zinc finger CCCH domain-containing protein 63 (S/424) | 0.90 | 0.93 | 1.38 | 0.93 |
ROC1 Homeobox-leucine zipper protein ROC1 (ROC1/S/46) | 1.01 | 1.08 | 4.20 | 0.40 |
DNA binding protein, mRNA (S/176) | 0.94 | 0.92 | 0.75 | 1.28 |
DNA binding protein, mRNA (S/204) | 0.8 | 0.92 | 1.33 | 1.07 |
DNA binding protein, mRNA (S/64) | 1.11 | 1.09 | 0.69 | 1.02 |
Transport | ||||
Hexose transporter (Hexose transporter/S/643) | 0.55 | 0.64 | 2.11 | 0.86 |
Hexose transporter (Hexose transporter/S/70) | 0.61 | 0.75 | 1.78 | 0.97 |
Putative sulfate transporter (S/643) | 0.68 | 0.96 | 1.90 | 0.68 |
Putative sulfate transporter (Putative sulfate transporter/S/651) | 0.73 | 0.9 | 1.69 | 0.82 |
Sugar transport1 isoform 1 (Sugar transport1 isoform 1/S/33) | 0.66 | 0.77 | 1.56 | 0.66 |
Sugar transport1 isoform 1 (Sugar transport1 isoform 1/S/32) | 0.84 | 0.98 | 1.31 | 0.75 |
Sugar transport1 isoform 1 (Sugar transport1 isoform 1/S/485) | 1.24 | 1.32 | 0.65 | 1.16 |
Amino acid permease-like (Amino acid permease-like/S/20) | 0.22 | 0.47 | 0.98 | 2.45 |
Amino acid permease-like (Amino acid permease-like/S/47) | 1.10 | 1.25 | 0.61 | 1.03 |
Amino acid permease-like (Amino acid permease-like/Y/49) | 1.25 | 1.22 | 0.52 | 1.02 |
Amino acid permease-like (Amino acid permease-like /T/41) | 1.26 | 1.23 | 0.47 | 1.01 |
Amino acid permease-like (Amino acid permease-like/S/42) | 1.28 | 1.22 | 0.45 | 1.01 |
Calcium-transporting ATPase 1, plasma membrane-type (S/45) | 0.87 | 1.17 | 0.73 | 1.15 |
MATE efflux family protein (MATE efflux family protein/S/12) | 1.10 | 1.07 | 0.65 | 1.01 |
ABC transporter family protein, putative, expressed (S/99) | 0.69 | 0.89 | 2.43 | 0.61 |
ABC transporter family protein, putative, expressed (S/7) | 0.70 | 0.92 | 2.36 | 0.61 |
ABC transporter family protein, putative, expressed (S/63) | 0.81 | 0.72 | 2.30 | 0.72 |
ABC-2 type transporter family protein (S/617) | 0.75 | 0.80 | 1.53 | 1.13 |
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Wang, N.; Zhu, T.; Lu, N.; Wang, Z.; Yang, G.; Qu, G.; Kong, L.; Zhang, S.; Ma, W.; Wang, J. Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii. Int. J. Mol. Sci. 2019, 20, 1895. https://doi.org/10.3390/ijms20081895
Wang N, Zhu T, Lu N, Wang Z, Yang G, Qu G, Kong L, Zhang S, Ma W, Wang J. Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii. International Journal of Molecular Sciences. 2019; 20(8):1895. https://doi.org/10.3390/ijms20081895
Chicago/Turabian StyleWang, Nan, Tianqing Zhu, Nan Lu, Zhi Wang, Guijuan Yang, Guanzheng Qu, Lisheng Kong, Shougong Zhang, Wenjun Ma, and Junhui Wang. 2019. "Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii" International Journal of Molecular Sciences 20, no. 8: 1895. https://doi.org/10.3390/ijms20081895