Changes of Circulating Extracellular Vesicles from the Liver after Roux-en-Y Bariatric Surgery
Abstract
:1. Introduction
2. Results
3. Discussion
Limitations
4. Materials and Methods
4.1. Study Population
4.2. Laboratory Parameter Determination
4.3. Isolation of Extracellular Vesicles
4.4. Determination of Extracellular Vesicles
4.5. ELISA Determination of Annexin V+ Vesicles
4.6. RNA Isolation and qPCR
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Total Patients | 27 | |
Male | 6 (22%) | |
Female | 21 (78%) | |
Age | 43 ± 13 | |
Diabetes | 8 (30%) | |
Smoking | 8 (30%) | |
before surgery | one year after surgery | |
AST U/L | 28.44 ± 13.9 | 22.28 ± 12.3 (p = 0.054) |
ALT U/L | 42.96 ± 28.1 | 21.47 ± 11.6 (p < 0.001) |
GGT U/L | 62.96 ± 72.3 | 21.18 ± 45.4 (p < 0.001) |
HDL (mg/dL) | 48.9 ± 14.4 | 55.8 ± 5.3 (p = 0.013) |
LDL (mg/dL) | 103.2 ± 24 | 76.9 ± 20.5 (p < 0.001) |
CRP (mg/dL) | 0.924 ± 0.8 | 0.414 ± 1.1 (p < 0.001) |
PAI-1 (ng/mL) | 99 ± 14.6 | 86.7 ± 27 (p = 0.048) |
Medication | ||
Statins | 7 (26%) | 2 (8%) |
Antidiabetics | 6 (22%) | 2 (8%) |
Insulin | 3 (11%) | 2 (8%) |
ACE inhibitors | 14 (52%) | 8 (30%) |
Beta blocker | 6 (22%) | 2 (8%) |
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Rega-Kaun, G.; Ritzel, D.; Kaun, C.; Ebenbauer, B.; Thaler, B.; Prager, M.; Demyanets, S.; Wojta, J.; Hohensinner, P.J. Changes of Circulating Extracellular Vesicles from the Liver after Roux-en-Y Bariatric Surgery. Int. J. Mol. Sci. 2019, 20, 2153. https://doi.org/10.3390/ijms20092153
Rega-Kaun G, Ritzel D, Kaun C, Ebenbauer B, Thaler B, Prager M, Demyanets S, Wojta J, Hohensinner PJ. Changes of Circulating Extracellular Vesicles from the Liver after Roux-en-Y Bariatric Surgery. International Journal of Molecular Sciences. 2019; 20(9):2153. https://doi.org/10.3390/ijms20092153
Chicago/Turabian StyleRega-Kaun, Gersina, Dorothea Ritzel, Christoph Kaun, Benjamin Ebenbauer, Barbara Thaler, Manfred Prager, Svitlana Demyanets, Johann Wojta, and Philipp J. Hohensinner. 2019. "Changes of Circulating Extracellular Vesicles from the Liver after Roux-en-Y Bariatric Surgery" International Journal of Molecular Sciences 20, no. 9: 2153. https://doi.org/10.3390/ijms20092153