Physical Activity Dynamically Regulates the Hippocampal Proteome along the Dorso-Ventral Axis
Abstract
:1. Introduction
2. Results
2.1. EPA Differently Impacts Adult Neurogenesis in Dorsal and Ventral Hippocampus
2.2. Label Free Quantitative Mass Spectrometry Detects Proteomic Alterations during Neurogenesis
2.3. Dorsal and Ventral Hippocampal Subregions Respond Differently to EPA
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Immunohistochemistry
4.2.1. Tissue Preparation
4.2.2. Tissue Selection
4.2.3. Imaging
4.2.4. Quantification
4.3. Proteomics
4.3.1. Tissue Preparation and Microdissection
4.3.2. Mass Spectrometry Analysis
4.3.3. Data Analysis
4.4. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CA | Cornu Ammonis |
DCX | Doublecortin |
DG | Dentate gyrus |
EPA | Enhanced physical activity |
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Frey, S.; Schieweck, R.; Forné, I.; Imhof, A.; Straub, T.; Popper, B.; Kiebler, M.A. Physical Activity Dynamically Regulates the Hippocampal Proteome along the Dorso-Ventral Axis. Int. J. Mol. Sci. 2020, 21, 3501. https://doi.org/10.3390/ijms21103501
Frey S, Schieweck R, Forné I, Imhof A, Straub T, Popper B, Kiebler MA. Physical Activity Dynamically Regulates the Hippocampal Proteome along the Dorso-Ventral Axis. International Journal of Molecular Sciences. 2020; 21(10):3501. https://doi.org/10.3390/ijms21103501
Chicago/Turabian StyleFrey, Surina, Rico Schieweck, Ignasi Forné, Axel Imhof, Tobias Straub, Bastian Popper, and Michael A. Kiebler. 2020. "Physical Activity Dynamically Regulates the Hippocampal Proteome along the Dorso-Ventral Axis" International Journal of Molecular Sciences 21, no. 10: 3501. https://doi.org/10.3390/ijms21103501