Otoprotective Effects of Zingerone on Cisplatin-Induced Ototoxicity
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animal Treatments
4.2. Histological Examination
4.3. Quantitative RT-PCR Analysis
4.4. Western Blot Analysis
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Primer Sequence (Forward) | Primer Sequence (Reverse) | Annealing Temperature (°C) | Product Size (bp) | RefSeq Number |
---|---|---|---|---|---|
CYP1A1 | 5’- CATCCCCCACAGCACCATAA -3’ | 5’- TTCGCTTGCCCAAACCAAAG -3’ | 60 | 212 | NM_012540.2 |
CYP1B1 | 5’- TGCTACTCGTTTCGGTCCTG -3’ | 5’- CAAGGCGAGCGAAGTACAAG -3’ | 60 | 162 | NM_012940.2 |
iNOS | 5’- AGGCCACCTCGGATATCTCT -3’ | 5’- TCTCTGGGTCCTCTGGTCAA -3’ | 60 | 85 | NM_012611.3 |
NFκB | 5’- TGTCTGCACCTGTTCCAAAGA-3’ | 5’- TGCCAGGTCTGTGAACACTC-3’ | 60 | 143 | NM_199267.2 |
IL6 | 5’- AGAGACTTCCAGCCAGTTGC-3’ | 5’- TGAAGTCTCCTCTCCGGACT-3’ | 60 | 88 | NM_012589.2 |
TNFα | 5’- CGTCAGCCGATTTGCCATTT -3’ | 5’- TCCCTCAGGGGTGTCCTTAG -3’ | 60 | 88 | NM_012675.3 |
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Lee, C.H.; Lee, D.-h.; Lee, S.M.; Kim, S.Y. Otoprotective Effects of Zingerone on Cisplatin-Induced Ototoxicity. Int. J. Mol. Sci. 2020, 21, 3503. https://doi.org/10.3390/ijms21103503
Lee CH, Lee D-h, Lee SM, Kim SY. Otoprotective Effects of Zingerone on Cisplatin-Induced Ototoxicity. International Journal of Molecular Sciences. 2020; 21(10):3503. https://doi.org/10.3390/ijms21103503
Chicago/Turabian StyleLee, Chang Ho, Da-hye Lee, So Min Lee, and So Young Kim. 2020. "Otoprotective Effects of Zingerone on Cisplatin-Induced Ototoxicity" International Journal of Molecular Sciences 21, no. 10: 3503. https://doi.org/10.3390/ijms21103503