Functional Block of Interleukin-6 Reduces a Bone Pain Marker But Not Bone Loss in Hindlimb-Unloaded Mice
Abstract
:1. Introduction
2. Results
2.1. Measurement of Pain-Related Behavior with Von Frey Filaments
2.2. Immunohistochemical Analysis in the DRGs
2.3. Analysis of Three-Dimensional Bone Structure by Micro-Computed Tomography (μCT)
2.4. Histological Analysis of Hindlimb Bone
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animals
4.3. Hindlimb Unloading
4.4. Experimental Protocol
4.5. Measurement of Pain-Related Behavior with Von Frey Filaments
4.6. Analysis of Three-Dimensional Bone Structure by μCT
4.7. Immunohistochemical Analysis of Hindlimb Bone and DRG
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BP | bisphosphonate |
BMDMs | bone marrow-derived macrophages |
BV | bone volume |
CGRP | calcitonin gene-related peptide |
DRG | dorsal root ganglion |
HL | hindlimb loaded |
HU | hindlimb unloading |
IVD | intervertebral disc |
μCT | micro-computed tomography |
TRAP | tartrate-resistant acid phosphatase |
Tb.N | trabecular number |
Tb.Sp | trabecular separation |
Tb.Th | trabecular thickness |
TRPV1 | transient receptor potential channel vanilloid subfamily member 1 |
TV | tissue volume |
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Wakabayashi, H.; Miyamura, G.; Nagao, N.; Kato, S.; Naito, Y.; Sudo, A. Functional Block of Interleukin-6 Reduces a Bone Pain Marker But Not Bone Loss in Hindlimb-Unloaded Mice. Int. J. Mol. Sci. 2020, 21, 3521. https://doi.org/10.3390/ijms21103521
Wakabayashi H, Miyamura G, Nagao N, Kato S, Naito Y, Sudo A. Functional Block of Interleukin-6 Reduces a Bone Pain Marker But Not Bone Loss in Hindlimb-Unloaded Mice. International Journal of Molecular Sciences. 2020; 21(10):3521. https://doi.org/10.3390/ijms21103521
Chicago/Turabian StyleWakabayashi, Hiroki, Gaku Miyamura, Nobuto Nagao, Sho Kato, Yohei Naito, and Akihiro Sudo. 2020. "Functional Block of Interleukin-6 Reduces a Bone Pain Marker But Not Bone Loss in Hindlimb-Unloaded Mice" International Journal of Molecular Sciences 21, no. 10: 3521. https://doi.org/10.3390/ijms21103521